CoraLite® Plus 488-conjugated PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4H4D1

Cat no : CL488-66220

Synonyms

CD279, hPD 1, hPD l, PD 1, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-66220

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66220 (PD-1/CD279 antibody), at dilution of 1:150.

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  FC experiment of Jurkat using CL488-66220

  1X10^6 Jurkat cells untreated (blue, dashed line) or treated with CD3 antibody (OKT3, 2 ug/ml) were surface stained with 0.4 ug CoraLite® Plus 488 Anti-Human PD-1/CD279 (CL488-66220, Clone: 4H4D1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue, solid line). Cells were not fixed.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue
• Positive FC detected in: CD3 antibody treated Jurkat cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66220 targets PD-1/CD279 in IF, FC applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PDCD1
• Gene ID (NCBI): 5133
• RRID: AB_2883287
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438). This anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.