Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human appendicitis tissue, human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue, Raji cells |
| Positive IF/ICC detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24828-1-AP targets CD20 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17309 Product name: Recombinant human MS4A1,CD20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 141-297 aa of BC002807 Sequence: IKISHFLKMESLNFIRAHTPYINIYNCEPANPSEKNSPSTQYCYSIQSLFLGILSVMLIFAFFQELVIAGIVENEWKRTCSRPKSNIVLLSAEEKKEQTIEIKEEVVGLTETSSQPKNEEDIEIIPIQEEEEEETETNFPEPPQDQESSPIENDSSP Predict reactive species |
| Full Name | membrane-spanning 4-domains, subfamily A, member 1 |
| Calculated Molecular Weight | 297 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC002807 |
| Gene Symbol | CD20 |
| Gene ID (NCBI) | 931 |
| RRID | AB_2879747 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11836 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD20 antibody 24828-1-AP | Download protocol |
| IHC protocol for CD20 antibody 24828-1-AP | Download protocol |
| IP protocol for CD20 antibody 24828-1-AP | Download protocol |
| WB protocol for CD20 antibody 24828-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD20 Polyclonal antibody (Cat# 24828-1-AP) has 12 citations spanning journals including Cancer Research, Journal for ImmunoTherapy of Cancer, British Journal of Pharmacology, Acta Physiologica, International Journal of Molecular Sciences, Lung Cancer, Clinical and Experimental Immunology, Pathology Research and Practice, Experimental Cell Research, Frontiers in Oncology, Technology in Cancer Research & Treatment, and Heliyon. Research fields cover oncology (gastric, lung, colorectal, breast, ovarian, pancreatic, and lymphoma cancers), immunology (Sjögren's syndrome, immune infiltration), pharmacology (ulcerative colitis), and radioimmunotherapy.
| Species | Application | Title |
|---|---|---|
Cancer Res SIRT1 Inhibits T-cell Infiltration and Tertiary Lymphoid Structure Formation to Promote Radioimmunotherapy Resistance. | ||
J Immunother Cancer Infiltrating pattern and prognostic value of tertiary lymphoid structures in resected non-functional pancreatic neuroendocrine tumors. | ||
Br J Pharmacol Tryptophan 2,3-dioxygenase deficiency attenuates dextran sodium sulphate -induced ulcerative colitis in mice by suppressing macrophage polarisation and reducing intestinal epithelial cell apoptosis. | ||
Acta Physiol (Oxf) Acute physical exercise and long-term individual shear rate therapy increase telomerase activity in human peripheral blood mononuclear cells. | ||
Int J Mol Sci CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing | ||
Lung Cancer The survival analysis of stage III and IV inoperable lung large cell neuroendocrine carcinoma and the role of LIPI in immunological stratification |
Reviews
What customers say
The single review for this product reports a 4/5 rating. The user found it effective for immunofluorescence on FFPE prostate tissue at a 1:50 dilution, using Tris-EDTA (pH 9) for antigen retrieval. The antibody also performed well in IHC with sodium citrate antigen retrieval. Overall, the feedback is positive, confirming reliable performance across both IF and IHC applications on formalin-fixed paraffin-embedded samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emma (Verified Customer) (11-29-2021) | Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9. Also works by IHC with sodium citrate antigen retrieval.
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