Anti-Mouse PD-L2/CD273 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98241-1-RR

  1x10^6 untreated or GM-CSF treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L2/CD273 Rabbit Recombinant Antibody (98241-1-RR, Clone: 241728D8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11c (N418) (APC-65130, Clone: N418). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98241-1-RR

  1x10^6 GM-CSF treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L2/CD273 Rabbit Recombinant Antibody (98241-1-RR, Clone:241728D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L2/CD273 Rabbit Recombinant Antibody (98241-1-RR, Clone:241728D8) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

PD-L2/CD273 Recombinant Antibody for FC

Cat No. 98241-1-RR

Clone No.241728D8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD273, Butyrophilin B7-DC, Btdc, B7-DC, B7dc

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Tested Applications

• Positive FC detected in: GM-CSF treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98241-1-RR targets PD-L2/CD273 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: programmed cell death 1 ligand 2
• Calculated Molecular Weight: 28kDa
• GenBank Accession Number: NM_021396.2
• Gene Symbol: Pdcd1lg2
• Gene ID (NCBI): 58205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9WUL5
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.