FITC Plus Anti-Mouse PD-L2/CD273 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using FITC-98241

  1x10^6 untreated or GM-CSF treated mouse splenocytes were surface stained with APC Anti-Mouse CD11c and 0.25 ug FITC Plus Anti-Mouse PD-L2/CD273 Rabbit RecAb (FITC-98241, Clone: 241728D8). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-98241

  1x10^6 GM-CSF treated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse PD-L2/CD273 Rabbit RecAb (FITC-98241, Clone: 241728D8) (red) or FITC Plus Rabbit IgG Isotype Control RecAb (FITC-98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse PD-L2/CD273 Rabbit RecAb (FITC-98241, Clone: 241728D8) (black, dashed). Cells were not fixed.

PD-L2/CD273 Uni-rAb^TM Recombinant Antibody for FC

Cat No. FITC-98241

Clone No.241728D8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

PD-L2/B7-DC, 241728D8, B7dc, B7-DC, Btdc

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Tested Applications

• Positive FC detected in: GM-CSF treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98241 targets PD-L2/CD273 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PD-L2/CD273 fusion protein Eg1842
• Full Name: programmed cell death 1 ligand 2
• Calculated Molecular Weight: 28kDa
• GenBank Accession Number: NM_021396.2
• Gene Symbol: Pdcd1lg2
• Gene ID (NCBI): 58205
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9WUL5
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages (PMID: 14515254). PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T cell activation in the periphery.