PDCD5 Polyclonal antibody

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  WB analysis of human heart using 12456-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12456-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12456-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12456-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PDCD5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12456-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

PDCD5, programmed cell death 5, Protein TFAR19, TFAR19

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Tested Applications

• Positive WB detected in: human heart tissue, PC-3 cells, human brain tissue, A549 cells
• Positive IHC detected in: human endometrial cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12456-1-AP targets PDCD5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCD5 fusion protein Ag3122
• Full Name: programmed cell death 5
• Calculated Molecular Weight: 125 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC015519
• Gene Symbol: PDCD5
• Gene ID (NCBI): 9141
• RRID: AB_2162450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14737
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDCD5, also called TFAR19 (TF1 cell apoptosis-related gene 19), was first identified as a gene up-regulated in TF-1 cells under-going apoptosis (PMID: 9920759). PDCD5 can promote pro-grammed cell death in different cell types in response to various stimuli and also enhance TAJ/TROY-induced paraptosis-like cell death (PMID: 15020679). During apoptosis, PDCD5 is rapidly upregulated and translocates from the cytoplasm to nucleus (PMID: 11741587). PDCD5 is a positive regulator of Tip60 and also has a potential ability to interact with p53 (PMID: 22914926, PMID: 19308289). Recent studies have also revealed that PDCD5 may be a suppressor gene and expressed at lower levels in many cancers, including hepatocellular carcinoma, breast cancer, gastric cancer, cervical cancer, lung cancer, astrocytic gliomas, and in leukemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC, WB	Nat Commun; Club cell-specific role of programmed cell death 5 in pulmonary fibrosis.; Authors - Soo-Yeon Park
	WB	Nat Commun; Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.; Authors - Hyo-Kyoung Choi ; KD Validated
human	WB	J Nanobiotechnology; Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.; Authors - Lin Lin
human	WB	J Cell Physiol; Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.; Authors - Dong Lin
human	WB	Sci Rep; Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.; Authors - Soo-Yeon Park
human	WB, IF	Cancer Lett; DNAJB1 destabilizes PDCD5 to suppress p53-mediated apoptosis.; Authors - Xiandan Cui