PDCD5 Polyclonal antibody

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  WB analysis of human heart using 12456-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12456-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12456-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12456-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12456-1-AP (PDCD5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12456-1-AP (PDCD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 12456-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12456-1-AP (PDCD5 Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PDCD5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12456-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Synonyms

PDCD5, programmed cell death 5, Protein TFAR19, TFAR19

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Tested Applications

• Positive WB detected in: human heart tissue, PC-3 cells, human brain tissue, A549 cells
• Positive IHC detected in: human endometrial cancer tissue, human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

12456-1-AP targets PDCD5 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCD5 fusion protein Ag3122
• Full Name: programmed cell death 5
• Calculated Molecular Weight: 125 aa, 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC015519
• Gene Symbol: PDCD5
• Gene ID (NCBI): 9141
• RRID: AB_2162450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14737
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDCD5, also called TFAR19 (TF1 cell apoptosis-related gene 19), was first identified as a gene up-regulated in TF-1 cells under-going apoptosis (PMID: 9920759). PDCD5 can promote pro-grammed cell death in different cell types in response to various stimuli and also enhance TAJ/TROY-induced paraptosis-like cell death (PMID: 15020679). During apoptosis, PDCD5 is rapidly upregulated and translocates from the cytoplasm to nucleus (PMID: 11741587). PDCD5 is a positive regulator of Tip60 and also has a potential ability to interact with p53 (PMID: 22914926, PMID: 19308289). Recent studies have also revealed that PDCD5 may be a suppressor gene and expressed at lower levels in many cancers, including hepatocellular carcinoma, breast cancer, gastric cancer, cervical cancer, lung cancer, astrocytic gliomas, and in leukemia.

Publications

Species	Application	Title
	IHC, WB	Nat Commun; Club cell-specific role of programmed cell death 5 in pulmonary fibrosis.; Authors - Soo-Yeon Park
	WB	Nat Commun; Programmed cell death 5 mediates HDAC3 decay to promote genotoxic stress response.; Authors - Hyo-Kyoung Choi ; KD Validated
human	WB	J Nanobiotechnology; Multifunctional photodynamic/photothermal nano-agents for the treatment of oral leukoplakia.; Authors - Lin Lin
human	WB	J Cell Physiol; Thyroid stimulating hormone aggravates diabetic retinopathy through the mitochondrial apoptotic pathway.; Authors - Dong Lin
human	WB	Sci Rep; Protein serine/threonine phosphatase PPEF-1 suppresses genotoxic stress response via dephosphorylation of PDCD5.; Authors - Soo-Yeon Park
human	WB, IF	Cancer Lett; DNAJB1 destabilizes PDCD5 to suppress p53-mediated apoptosis.; Authors - Xiandan Cui