PDE1A Polyclonal antibody

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  WB analysis using 12442-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12442-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12442-2-AP (PDE1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12442-2-AP

  IP result of anti-PDE1A (IP:12442-2-AP, 4ug; Detection:12442-2-AP 1:1000) with mouse brain tissue lysate 2080 ug.

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12442-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12442-2-AP (PDE1A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PDE1A Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12442-2-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

61 kDa Cam-PDE, Cam-PDE 1A, Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A, EC:3.1.4.17, hCam 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

12442-2-AP targets PDE1A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3157

  Product name: Recombinant human PDE1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 185-545 aa of BC022480

  Sequence: TRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQGESDLHKNSEDLVNAEEKHDETHS

• Full Name: phosphodiesterase 1A, calmodulin-dependent
• Calculated Molecular Weight: 545 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC022480
• Gene Symbol: PDE1A
• Gene ID (NCBI): 5136
• RRID: AB_2162893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P54750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE1A(Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin(PMID:12135876).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Biol Reprod; Luteinizing Hormone Causes Phosphorylation and Activation of the cGMP Phosphodiesterase PDE5 in Rat Ovarian Follicles, Contributing, Together with PDE1 Activity, to the Resumption of Meiosis.; Authors - Jeremy R Egbert
mouse	WB	Biol Reprod; Follicle-stimulating hormone and luteinizing hormone increase Ca2+ in the granulosa cells of mouse ovarian follicles†.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Generation and phenotypic characterization of Pde1a mutant mice.; Authors - Xiaofang Wang ; KO Validated
		Cell Rep; Single-Cell Analysis of Foxp1-Driven Mechanisms Essential for Striatal Development.; Authors - Ashley G Anderson
human	IHC	Front Genet; Transcriptome profiling of intact bowel wall reveals that PDE1A and SEMA3D are possible markers with roles in enteric smooth muscle apoptosis, proliferative disorders, and dysautonomia in Crohn's disease; Authors - Yun Yang
human	WB	Mol Cancer; Therapy-induced senescence is a transient drug resistance mechanism in breast cancer; Authors - Eszter Bajtai