PDE1A Recombinant monoclonal antibody

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  WB analysis using 87154-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87154-1-RR (PDE1A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  Affinity of 87154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87154-1-RR against Human PDE1A were performed. The affinity constant is below 1 pM.

PDE1A Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87154-1-RR

Clone No.252285B6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

61 kDa Cam-PDE, Cam-PDE 1A, Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A, EC:3.1.4.17, hCam 1

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87154-1-RR targets PDE1A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3157

  Product name: Recombinant human PDE1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 185-545 aa of BC022480

  Sequence: TRYDLINRFKIPVSCLITFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNILINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWKLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIVIPLIEEASKAETSSYVASSSTTIVGLHIADALRRSNTKGSMSDGSYSPDYSLAAVDLKSFKNNLVDIIQQNKERWKELAAQGESDLHKNSEDLVNAEEKHDETHS

• Full Name: phosphodiesterase 1A, calmodulin-dependent
• Calculated Molecular Weight: 545 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC022480
• Gene Symbol: PDE1A
• Gene ID (NCBI): 5136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P54750
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE1A (Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A) is also named as Cam-PDE 1A, 61 kDa Cam-PDE, hCam-1 and belongs to the cyclic nucleotide phosphodiesterase (PDEs) family. The PDEs play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. This protein has 9 isoforms produced by alternative splicing with the molecular weight from 57 kDa to 63 kDa. There is a report showing the major 67-kD PDE1A isoform identified in human sperm associates tightly with calmodulin and is not activated by Ca(2+)/calmodulin (PMID:12135876).