PDE2A Polyclonal antibody

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  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 55306-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55306-1-AP

  IP result of anti-PDE2A (IP:55306-1-AP, 4ug; Detection:55306-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PDE2A Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 55306-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CGS PDE, cGSPDE, PDE2A, PDE2A1, PED2A4

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

55306-1-AP targets PDE2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: phosphodiesterase 2A, cGMP-stimulated
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 100-106 kDa
• GenBank Accession Number: NM_002599
• Gene Symbol: PDE2A
• Gene ID (NCBI): 5138
• RRID: AB_11182279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00408
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
rat	WB	Br J Pharmacol; 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner.; Authors - Sofya Pozdniakova
rat	WB	Biochem Pharmacol; Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A.; Authors - Módis Katalin K
rat	WB	Front Pharmacol; Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.; Authors - Xiaofei Zhu
human,mouse	WB,IF	Commun Biol; Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.; Authors - Miguel J Lobo ; KO Validated
rat	WB	JCI Insight; Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity.; Authors - Kun Liu