PDE2A Polyclonal antibody

• Featured Product
• KD/KO Validated

PDE2A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 55306-1-AP

Synonyms

CGS PDE, cGSPDE, PDE2A, PDE2A1, PED2A4

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55306-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55306-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55306-1-AP (PDE2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 55306-1-AP

  IP result of anti-PDE2A (IP:55306-1-AP, 4ug; Detection:55306-1-AP 1:500) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55306-1-AP (PDE2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55306-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55306-1-AP (PDE2A Antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

55306-1-AP targets PDE2A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphodiesterase 2A, cGMP-stimulated
• Calculated Molecular Weight: 106 kDa
• Observed Molecular Weight: 100-106 kDa
• GenBank Accession Number: NM_002599
• Gene Symbol: PDE2A
• Gene ID (NCBI): 5138
• RRID: AB_11182279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE2A, also named as cGSPDE and CGS-PDE, belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. It is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. This antibody is specific to PDE2A.

Publications

Species	Application	Title
mouse	WB	Sci Adv; RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease.; Authors - Mehdi Eshraghi
rat	WB	Br J Pharmacol; 17β-estradiol reduces mitochondrial cAMP content and cytochrome oxidase activity in a phosphodiesterase 2-dependent manner.; Authors - Sofya Pozdniakova
rat	WB	Biochem Pharmacol; Hydrogen sulfide-mediated stimulation of mitochondrial electron transport involves inhibition of the mitochondrial phosphodiesterase 2A, elevation of cAMP and activation of protein kinase A.; Authors - Módis Katalin K
rat	WB	Front Pharmacol; Proteomics Study Reveals the Anti-Depressive Mechanisms and the Compatibility Advantage of Chaihu-Shugan-San in a Rat Model of Chronic Unpredictable Mild Stress.; Authors - Xiaofei Zhu
human,mouse	WB,IF	Commun Biol; Phosphodiesterase 2A2 regulates mitochondria clearance through Parkin-dependent mitophagy.; Authors - Miguel J Lobo ; KO Validated
rat	WB	JCI Insight; Phosphodiesterase 2A as a therapeutic target to restore cardiac neurotransmission during sympathetic hyperactivity.; Authors - Kun Liu