PDGF-D Polyclonal antibody

• Featured Product
• KD/KO Validated

PDGF-D Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14075-1-AP

Synonyms

PDGFD, Platelet-derived growth factor D, latent form, Platelet-derived growth factor D, PDGFD receptor binding form, PDGF D

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Validation Data Gallery

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  IHC staining of human prostate cancer using 14075-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 14075-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 14075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 14075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 14075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Product Information

14075-1-AP targets PDGF-D in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGF-D fusion protein Ag5154
• Full Name: platelet derived growth factor D
• Calculated Molecular Weight: 43 kDa
• GenBank Accession Number: BC030645
• Gene Symbol: PDGFD
• Gene ID (NCBI): 80310
• RRID: AB_2162208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell; Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor.; Authors - Alexander D Barrow
human	IHC,IF	Cell Oncol (Dordr); Exploring the impact of PDGFD in osteosarcoma metastasis through single-cell sequencing analysis; Authors - Yujing Huang
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
human	IHC	J Immunol; Cutting Edge: PDGF-DD Binding to NKp44 Costimulates TLR9 Signaling and Proinflammatory Cytokine Secretion in Human Plasmacytoid Dendritic Cells; Authors - Alexander David Barrow
mouse	WB	Front Pharmacol; M2 Macrophages Promote PDGFRβ+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.; Authors - Ziyu Li
rat	IHC	ACS Omega; Promoted Skin Wound Healing by Tail-Amputated Eisenia foetida Proteins via the Ras/Raf/MEK/ERK Signaling Pathway; Authors - Tianyi Li