Tested Applications
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14075-1-AP targets PDGF-D in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5154 Product name: Recombinant human PDGFD protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-364 aa of BC030645 Sequence: MHRLIFVYTLICANFCSCRDTSATPQSASIKALRNANLRRDDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR Predict reactive species |
| Full Name | platelet derived growth factor D |
| Calculated Molecular Weight | 43 kDa |
| GenBank Accession Number | BC030645 |
| Gene Symbol | PDGFD |
| Gene ID (NCBI) | 80310 |
| RRID | AB_2162208 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-derived growth factor-D (PDGF-D) is the newest member of the PDGF family. It is secreted as a latent dimer that must undergo extracellular proteolytic removal of its N-terminal CUB domain before the growth-factor domain can activate the β-PDGFR. Once activated, PDGF-D drives proliferation, migration and survival of mesenchymal cells and promotes angiogenesis, wound healing and tissue remodeling. Clinically, PDGF-D is up-regulated in several cancers and in fibrotic diseases of the heart, kidney and lung, making it a potential therapeutic target.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDGF-D antibody 14075-1-AP | Download protocol |
| IHC protocol for PDGF-D antibody 14075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PDGFD antibody (Cat# 14075-1-AP) has 22 total citations published in journals including Cell, J Immunol, FASEB J, ACS Omega, Front Pharmacol, J Invest Dermatol, Cell Oncology, Nutrients, Brain Res, Toxicol Lett, and others. Associated research fields encompass tumor angiogenesis, wound healing, fibrosis (liver, peritoneal, renal), spinal cord injury, neuropathic pain, corneal neovascularization, breast/pancreatic/hepatocellular cancers, immune cell biology, and reproductive physiology. Applications span WB, IHC, and IF across human, mouse, rat, and goat species.
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Photocrosslinked carboxymethylcellulose-based hydrogels: Synthesis, characterization for curcumin delivery and wound healing | ||
J Invest Dermatol Identifying Potential Drug Targets for Keloid: A Mendelian Randomization Study. | ||
Nutrients Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats. | ||
Cell Oncol (Dordr) Exploring the impact of PDGFD in osteosarcoma metastasis through single-cell sequencing analysis | ||
Front Pharmacol M2 Macrophages Promote PDGFRβ+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway. |
