PDGFD Polyclonal antibody

PDGFD Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14075-1-AP

Synonyms

IEGF, Iris expressed growth factor, MSTP036, PDGF D, PDGFD, PDGFD receptor binding form, SCDGF B, SCDGFB, UNQ1899/PRO4345

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Validation Data Gallery

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  IHC staining of human prostate cancer using 14075-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 14075-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14075-1-AP (PDGFD antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 14075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 14075-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PDGFD antibody (14075-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: rat brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

14075-1-AP targets PDGFD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFD fusion protein Ag5154
• Full Name: platelet derived growth factor D
• Calculated molecular weight: 43 kDa
• GenBank accession number: BC030645
• Gene symbol: PDGFD
• Gene ID (NCBI): 80310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Cell; Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor.; Authors - Alexander D Barrow
human	WB	Cell Physiol Biochem; Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level.; Authors - Qin Zheng
mouse	WB	Front Pharmacol; M2 Macrophages Promote PDGFRβ+ Pericytes Migration After Spinal Cord Injury in Mice via PDGFB/PDGFRβ Pathway.; Authors - Ziyu Li
rat	WB	Nutrients; Protective Effect of the Total Saponins from Rosa laevigata Michx Fruit against Carbon Tetrachloride-Induced Liver Fibrosis in Rats.; Authors - Deshi Dong
goat	IHC	Domest Anim Endocrinol; Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.; Authors - I R Brito
rat	IF	Brain Res; Suppression of PDGF Induces Neuronal Apoptosis after Neonatal Cerebral Hypoxia and Ischemia by Inhibiting P-PI3K and P-AKT signaling pathways.; Authors - Liu-Lin Xiong