PDI Monoclonal antibody

PDI Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E6A11

Cat no : 66422-1-Ig

Synonyms

DSI, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase



Tested Applications

Positive WB detected inHEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:10000-1:100000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

66422-1-Ig targets PDI in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PDI fusion protein Ag1747
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated molecular weight 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC014504
Gene symbol P4HB
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Protocols

Product Specific Protocols
WB protocol for PDI antibody 66422-1-IgDownload protocol
IHC protocol for PDI antibody 66422-1-IgDownload protocol
IF protocol for PDI antibody 66422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell Death Dis

A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.

Authors - Peng Wang
humanIF

Cell Signal

HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.

Authors - Liu Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tom (Verified Customer) (12-15-2020)

10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
PDI Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:66422-1-Ig)