PDI Monoclonal antibody

PDI Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, pig, mouse, rat and More (1)







Cat no : 66422-1-Ig


DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

Tested Applications

Positive WB detected inHEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:10000-1:100000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66422-1-Ig targets PDI in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PDI fusion protein Ag1747
Full Name prolyl 4-hydroxylase, beta polypeptide
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC014504
Gene Symbol PDI
Gene ID (NCBI) 5034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).


Product Specific Protocols
WB protocol for PDI antibody 66422-1-IgDownload protocol
IHC protocol for PDI antibody 66422-1-IgDownload protocol
IF protocol for PDI antibody 66422-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochim Biophys Acta Mol Cell Res

SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization

Authors - Wojciech Wiertelak

J Cell Sci

A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function

Authors - Janine McCaughey

Mol Pharm

Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization.

Authors - Xiaofan Jia

Cell Signal

HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.

Authors - Liu Liu

Biochim Biophys Acta Gen Subj

Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels

Authors - Tianmiao Huang

Mol Med Rep

Sigma‑1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β‑cells.

Authors - Mengting Ke


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (12-15-2020)

10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HEK293T
PDI Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:66422-1-Ig)