|Positive WB detected in||HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells|
|Positive IHC detected in||human liver tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:10000-1:100000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66422-1-Ig targets PDI in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|Tested Reactivity||human, pig, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||PDI fusion protein Ag1747|
|Full Name||prolyl 4-hydroxylase, beta polypeptide|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC014504|
|Gene ID (NCBI)||5034|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
Cell Death Dis
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (12-15-2020)
10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.