Tested Applications
| Positive WB detected in | HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells |
| Positive IHC detected in | human liver tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66422-1-Ig targets PDI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, bovine |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1747 Product name: Recombinant human PDI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 324-508 aa of NM_000918 Sequence: MTKYKPESEELTAEWITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDDQKAVKDEL Predict reactive species |
| Full Name | prolyl 4-hydroxylase, beta polypeptide |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | NM_000918 |
| Gene Symbol | PDI |
| Gene ID (NCBI) | 5034 |
| RRID | AB_2881794 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07237 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDI antibody 66422-1-Ig | Download protocol |
| IHC protocol for PDI antibody 66422-1-Ig | Download protocol |
| WB protocol for PDI antibody 66422-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDI antibody (Cat# 66422-1-Ig) has 29 citations published in journals including Neuron, Adv Sci, J Exp Clin Cancer Res, Nucleic Acids Res, FASEB J, J Virol, Cell Death Dis, Chin Herb Med, and others. Research fields span ER stress and protein quality control, cancer (colorectal, breast, hepatocellular), lipid metabolism, viral infections (SARS-CoV-2, enterovirus, reovirus), diabetes, atherosclerosis, embryonic development, and glycosylation.
| Species | Application | Title |
|---|---|---|
Exp Mol Med Cholesterol biosynthesis induced by radiotherapy inhibits cGAS-STING activation and contributes to colorectal cancer treatment resistance | ||
Neuron A gain-of-function Retsat variant from high-altitude adaptation promotes myelination via a neuronal dihydroretinoic acid-RXR-γ pathway | ||
Nucleic Acids Res IRE1α RNase activity is critical for early embryo development by degrading maternal transcripts | ||
J Exp Clin Cancer Res Intracellular lipid droplets positively regulate the dormancy of colorectal cancer cells depending on ELF1-ACSL5 signaling | ||
Adv Sci (Weinh) Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms. | ||
Adv Sci (Weinh) Aloe Emodin Alleviates Radiation-Induced Heart Disease via Blocking P4HB Lactylation and Mitigating Kynurenine Metabolic Disruption |
Reviews
What customers say
One reviewer (Tom R.) rated this antibody 5/5 stars for Western Blot on HEK293T lysate at a 1:5,000 dilution. The protocol used 10 µg total protein, 5% BSA blocking, overnight incubation at 4°C, and anti-mouse HRP secondary at 1:10,000. The result showed clear band detection, indicating reliable performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (12-15-2020) | 10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.
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