PDI Monoclonal antibody

PDI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

15

CloneNo.

2E6A11

Reviews (1)

Cat no : 66422-1-Ig

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

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Validation Data Gallery

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  WB analysis using 66422-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66422-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human small intestine using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 66422-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 66422-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:4059 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of HepG2 using 66422-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue, HepG2 cells, L02 cells, COLO 320 cells
• Positive IHC detected in: human liver tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:100000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 14 publications below

Product Information

66422-1-Ig targets PDI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• RRID: AB_2881794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Publications

Species	Application	Title
bovine	IF	J Agric Food Chem; Acylated Ghrelin Activates PI3K/mTOR Signaling Pathway by Promoting ThPOK Acetylation to Promote Milk Fat Synthesis in Bovine Mammary Epithelial Cells; Authors - Jiaxin Wang
human	IF	Biochim Biophys Acta Mol Cell Res; SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization; Authors - Wojciech Wiertelak
human	IF	J Cell Sci; A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function; Authors - Janine McCaughey
human	WB	Mol Pharm; Highly Efficient Method for Intracellular Delivery of Proteins Mediated by Cholera Toxin-Induced Protein Internalization.; Authors - Xiaofan Jia
human	IF	Cell Signal; HRD1-mediated PTEN degradation promotes cell proliferation and hepatocellular carcinoma progression.; Authors - Liu Liu
human	IF	Biochim Biophys Acta Gen Subj; Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels; Authors - Tianmiao Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (12-15-2020)

10ug total protein of HEK293T lysate loaded. Membrane blocked in 5% BSA. Antibody (1:5,000) incubated overnight in block at 4 degrees. Anti-mouse HRP used at 1 in 10,000 to detect band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK293T

PDI Antibody Western Blot validation (1:5000 dilution) in HEK293T (Cat no:66422-1-Ig)