PDI Monoclonal antibody, PBS Only

PDI Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E6A11

Cat no : 66422-1-PBS

Synonyms

Cell and organelle markers, DSI, endoplasmic reticulum marker, ERBA2L, GIT, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

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Validation Data Gallery

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  WB analysis using 66422-1-Ig (same clone as 66422-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66422-1-Ig (same clone as 66422-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66422-1-Ig (PDI Antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66422-1-Ig (PDI Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66422-1-Ig (same clone as 66422-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDI antibody (66422-1-Ig, Clone: 2E6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66422-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66422-1-PBS targets PDI in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: P4HB
• Gene ID (NCBI): 5034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).