CoraLite®594-conjugated PDI Monoclonal antibody

PDI Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2E6A11

Cat no : CL594-66422

Synonyms

Cell and organelle markers, DSI, endoplasmic reticulum marker, ER marker, ERBA2L, GIT, Mitochondria Marker, Mitochondrial Marker, P4HB, P4Hbeta, p55, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, Protein disulfide isomerase

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-66422

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 PDI antibody (CL594-66422, Clone: 2E6A11 ) at dilution of 1:200.

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  FC experiment of HeLa using CL594-66422

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human PDI (CL594-66422, Clone:2E6A11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66422 targets PDI in IF, FC (Intra) applications and shows reactivity with human, pig, mouse, rat samples.

• Tested Reactivity: human, pig, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: P4HB
• Gene ID (NCBI): 5034
• RRID: AB_2883581
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C