PEG10 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86928-4-RR (same clone as 86928-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86928-4-RR (PEG10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86928-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 86928-4-RR

  Biolayer interferometry (BLl) kinetic assays of 86928-4-RR against Human PEG10 were performed. The affinity constant is 2.61 nM.

PEG10 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86928-4-PBS

Clone No.251980A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

EDR, Embryonal carcinoma differentiation-regulated protein, HB 1, HB1, KIAA1051

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Product Information

86928-4-PBS targets PEG10 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5754

  Product name: Recombinant human PEG10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC050659

  Sequence: MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL

• Full Name: paternally expressed 10
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 50 kDa, 100 kDa
• GenBank Accession Number: BC050659
• Gene Symbol: PEG10
• Gene ID (NCBI): 23089
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q86TG7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Paternally expressed imprinted gene 10 (PEG10) is an evolutionarily conserved HERV gene belonging to the Ty3/Gypsy family of retrotransposons. The mRNA of PEG10 encodes at least two protein isoforms: the major PEG10 protein product reading frame 1 (RF1-PEG10, ~50 kDa) and reading frames 1 and 2 (RF1/RF2-PEG10, ~100 kDa), which are translated by a typical retroviral-1 ribosomal frameshift mechanism. PEG10 is strongly expressed in placenta, ovary and testis as well as somatic adult organs including brain, kidney and lung . Researches on mice suggest that PEG10 plays an important role in placenta formation and adipocyte differentiation, and its knockout can cause embryonic lethality.(PMID: 30123090, PMID: 32244497)