Tested Applications
| Positive WB detected in | THP-1 cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12053-1-AP targets PELI1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2683 Product name: Recombinant human PELI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-418 aa of BC011419 Sequence: MKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLD Predict reactive species |
| Full Name | pellino homolog 1 (Drosophila) |
| Calculated Molecular Weight | 418 aa, 46 kDa |
| Observed Molecular Weight | 46-53 kDa |
| GenBank Accession Number | BC011419 |
| Gene Symbol | PELI1 |
| Gene ID (NCBI) | 57162 |
| RRID | AB_2161180 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96FA3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PELI1 encodes pellino-1, an E3 ubiquitin-protein ligase pellino homolog. E3 ubiquitin ligase functions by catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Pellino-1 is involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Pellino-1 can mediate 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation thus playing a role in cellular inflammation. This antibody may cross react with Pellino-3.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PELI1 antibody 12053-1-AP | Download protocol |
| IP protocol for PELI1 antibody 12053-1-AP | Download protocol |
| WB protocol for PELI1 antibody 12053-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PELI1 antibody (Cat# 12053-1-AP) has 11 total citations across journals including Cell Death & Disease, PLoS Biology, FASEB Journal, Journal of Translational Medicine, Life Sciences, Cell Signaling, Autoimmunity, Lab Investigation, Cell Biology International, Global Spine Journal, and Journal of Steroid Biochemistry and Molecular Biology. Research fields span oncology (AML, pancreatic cancer, thyroid carcinoma), neuroinflammation, autoimmune diseases (SLE, premature ovarian insufficiency), diabetic kidney disease, sepsis-associated encephalopathy, spinal cord injury, gastric pathology, and ferroptosis in PCOS.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Cathepsin D promotes acute myeloid leukemia progression through stabilization of the anti-apoptotic proteins | ||
J Transl Med MiR-30c-5p loss-induced PELI1 accumulation regulates cell proliferation and migration via activating PI3K/AKT pathway in papillary thyroid carcinoma. | ||
PLoS Biol Peli1 impairs microglial Aβ phagocytosis through promoting C/EBPβ degradation.
| ||
Life Sci Lactylation-driven YTHDF3 promotes microglial pyroptosis in sepsis-associated encephalopathy via the Usp9x/NLRP3 Axis. | ||
Cell Signal PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation | ||
FASEB J Pellino1 Exacerbates Diabetic Kidney Disease Through NLRP3 Inflammasome Driven Pyroptosis in Renal Tubular Epithelial Cells.
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