PET117 Polyclonal antibody

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  WB analysis using 31066-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 31066-1-AP (PET117 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 31066-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 31066-1-AP (PET117 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 31066-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31066-1-AP (PET117 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PET117 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31066-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CSRP2BP, MC4DN1, PET117 Homolog

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31066-1-AP targets PET117 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34525

  Product name: Recombinant human PET117 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 23-81 aa of NX_Q6UWS5

  Sequence: VHVKQQWDQQRLRDGVIRDIERQIRKKENIRLLGEQIILTEQLEAEREKMLLAKGSQKS

• Full Name: Protein PET117 homolog, mitochondrial
• Calculated Molecular Weight: 81aa, 9 kDa
• Observed Molecular Weight: 10kDa
• GenBank Accession Number: NX_Q6UWS5
• Gene Symbol: PET117
• Gene ID (NCBI): 100303755
• RRID: AB_3669839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6UWS5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PET117 is a chaperone protein involved in complex IV assembly that plays a role in the regulation of mitochondria-encoded cytochrome c oxidase 1 (COX1) protein synthesis in human cells (PMID: 37247699).