Validation Data Gallery
|Positive WB detected in||A431 cells, HEK-293T cells, HeLa cells, SKOV-3 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
10594-1-AP targets PEX14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PEX14 fusion protein Ag0932|
|Full Name||peroxisomal biogenesis factor 14|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||57 kDa|
|GenBank accession number||BC006327|
|Gene ID (NCBI)||5195|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.
|Product Specific Protocols|
|WB protocol for PEX14 antibody 10594-1-AP||Download protocol|
|IHC protocol for PEX14 antibody 10594-1-AP||Download protocol|
|IF protocol for PEX14 antibody 10594-1-AP||Download protocol|
|IP protocol for PEX14 antibody 10594-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochim Biophys Acta
Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.
Lymphoblastoid cell lines for diagnosis of peroxisome biogenesis disorders.
Mol Biol Cell
Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5-dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics.
Methods Mol Biol
Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.
Cell Death Dis
A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.
An AMPKα2-specific phospho-switch controls lysosomal targeting for activation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tim (Verified Customer) (04-09-2021)
20ug cell lysate loaded and separated by SDS-PAGE prior to transfer. Antibody incubation overnight in cold room.
Tom (Verified Customer) (03-30-2021)
HEK293 cells fixed in methanol at -20 degrees. PEX14 antibody visualized using anti-rabbit 594 (red) secondary antibody.
Chun (Verified Customer) (12-05-2019)
A good antibody