PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (3)

Cat no : 10594-1-AP

Synonyms

NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein

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Validation Data Gallery

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  WB analysis using 10594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10594-1-AP

  IP Result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of HeLa using 10594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of COS7 cells using 10594-1-AP

  The antibody 10594-1-AP stain the Peroxisomes of the COS7 cell transfected with YFP-Lpx1. IF result in Paper with PMID:21741480, Dr. Sven Thoms.

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  IF Staining of NIH/3T3 using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PEX14 antibody (10594-1-AP) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of C2C12 using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using PEX14 antibody (10594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of HepG2 using 10594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol ) fixed HepG2 cells using 10594-1-AP (PEX14 Antibody, Green) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 66009-1-Ig (Beta actin antibody, Red) and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI, for Blue..

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  IF Staining of HepG2 using 10594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10594-1-AP (PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10594-1-AP

  1X10^6 HepG2 cells were stained with .2ug PEX14 antibody (10594-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 31 publications below

Product Information

10594-1-AP targets PEX14 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX14 fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated molecular weight: 41 kDa
• Observed molecular weight: 57 kDa
• GenBank accession number: BC006327
• Gene symbol: PEX14
• Gene ID (NCBI): 5195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Publications

Species	Application	Title
mouse	WB,IF	Biochim Biophys Acta; Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice.; Authors - Kovacs Werner J WJ
human	IF	JIMD Rep; Lymphoblastoid cell lines for diagnosis of peroxisome biogenesis disorders.; Authors - Grønborg Sabine S
human	IF	Mol Biol Cell; Novel regulatory roles of Mff and Drp1 in E3 ubiquitin ligase MARCH5-dependent degradation of MiD49 and Mcl1 and control of mitochondrial dynamics.; Authors - Edward Cherok
mouse	WB	Methods Mol Biol; Isolation of Peroxisomes from Mouse Brain Using a Continuous Nycodenz Gradient: A Comparison to the Isolation of Liver and Kidney Peroxisomes.; Authors - Miriam J Schönenberger
	IF	Cell Death Dis; A cross-talk between epithelium and endothelium mediates human alveolar-capillary injury during SARS-CoV-2 infection.; Authors - Peng Wang
mouse	WB	Cell Rep; An AMPKα2-specific phospho-switch controls lysosomal targeting for activation.; Authors - Kaitlin R Morrison

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tim (Verified Customer) (04-09-2021)

20ug cell lysate loaded and separated by SDS-PAGE prior to transfer. Antibody incubation overnight in cold room.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: HEK293

PEX14 Antibody Western Blot validation (1/2000 dilution) in HEK293 (Cat no:10594-1-AP)

FH

Tom (Verified Customer) (03-30-2021)

HEK293 cells fixed in methanol at -20 degrees. PEX14 antibody visualized using anti-rabbit 594 (red) secondary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293

PEX14 Antibody Immunofluorescence validation (1:100 dilution) in HEK293 (Cat no:10594-1-AP)

FH

Chun (Verified Customer) (12-05-2019)

A good antibody

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells

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