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PEX14 Antibody 19 Publications

Rabbit Polyclonal| Catalog number: 10594-1-AP

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Con: 50 μg/150 μl

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Tested Applications

Applications:
WB, IP, IIHC, IHC, IF, FC, ELISA
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Images See all

PEX14 Antibody WB A431 cells 10594-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours

PEX14 Antibody IF HepG2 cells 10594-1-AP

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 10594-1-AP(PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PEX14 Antibody IHC human liver cancer tissue 10594-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 10x lens)

PEX14 Antibody IHC human liver cancer tissue 10594-1-AP

Immunohistochemical of paraffin-embedded human liver cancer using 10594-1-AP(PEX14 antibody) at dilution of 1:50 (under 40x lens)

PEX14 Antibody IP mouse liver tissue 10594-1-AP

IP Result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

PEX14 Antibody FC HepG2 cells 10594-1-AP

1X10^6 HepG2 cells were stained with .2ug PEX14 antibody (10594-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Species specificity:
human, mouse, rat, monkey
Positive WB detected in:
A431 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human liver cancer tissue, human cervical cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, COS7 cells, HeLa cells
Positive FC detected in:
HepG2 cells
Recommended dilution:
WB : 1:2000-1:10000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PEX14 fusion protein Ag0932
Full name:
peroxisomal biogenesis factor 14
Calculated molecular weight:
41 kDa
Observed molecular weight:
57 kDa
GenBank accession number:
BC006327
Gene ID (NCBI):
5195
Gene symbol
PEX14
Synonyms
NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein

Background

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.


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