PEX14 Polyclonal antibody

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  WB analysis using 10594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10594-1-AP

  WB result of PEX14 antibody (10594-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.

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  IP experiment of mouse liver using 10594-1-AP

  IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse liver using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 10594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10594-1-AP (PEX14 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 10594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol ) fixed HepG2 cells using 10594-1-AP (PEX14 Antibody, Green) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 66009-1-Ig (Beta actin antibody, Red) and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI, for Blue..

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  IF Staining of HepG2 using 10594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 10594-1-AP (PEX14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of COS7 cells using 10594-1-AP

  The antibody 10594-1-AP stain the Peroxisomes of the COS7 cell transfected with YFP-Lpx1. IF result in Paper with PMID:21741480, Dr. Sven Thoms.

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  IF Staining of NIH/3T3 using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using PEX14 antibody (10594-1-AP) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of C2C12 using 10594-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using PEX14 antibody (10594-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  FC experiment of HeLa using 10594-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

PEX14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10594-1-AP

Publications(67)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

NAPP2, Peroxin 14, Peroxin-14, PEX, Pex14p

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, HeLa cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: mouse liver tissue, human liver cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 47 publications below

Product Information

10594-1-AP targets PEX14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, monkey, chicken, yeast
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX14 fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006327
• Gene Symbol: PEX14
• Gene ID (NCBI): 5195
• RRID: AB_2252194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75381
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Publications

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	IF	Cell; A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart.; Authors - José A Nicolás-Ávila
mouse	IF	Nat Cell Biol; Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties; Authors - Dennis K Jeppesen
human	IF	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty
mouse	IF	Mol Cell; Drp1 Tubulates the ER in a GTPase-Independent Manner.; Authors - Yoshihiro Adachi
mouse	WB,IHC	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this antibody on THP1 cells (20ug protein extract) at a 1:1000 dilution for WB and although I saw a band around the molecular weight of the protein, different bands were also detected suggesting some non-specific binding.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: THP1 cell line

PEX14 Antibody Western Blot validation (1:1000 dilution) in THP1 cell line (Cat no:10594-1-AP)

FH

Tim (Verified Customer) (04-09-2021)

20ug cell lysate loaded and separated by SDS-PAGE prior to transfer. Antibody incubation overnight in cold room.

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: HEK293

PEX14 Antibody Western Blot validation (1/2000 dilution) in HEK293 (Cat no:10594-1-AP)

FH

Tom (Verified Customer) (03-30-2021)

HEK293 cells fixed in methanol at -20 degrees. PEX14 antibody visualized using anti-rabbit 594 (red) secondary antibody.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293

PEX14 Antibody Immunofluorescence validation (1:100 dilution) in HEK293 (Cat no:10594-1-AP)

FH

Chun (Verified Customer) (12-05-2019)

A good antibody

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa cells