CoraLite®488-conjugated PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-10594

Synonyms

dJ734G22.2, NAPP2, Peroxin 14, PEX14, Pex14p, PTS1 receptor docking protein



Tested Applications

Positive IF detected inHeLa cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 10594-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

CL488-10594 targets PEX14 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX14 fusion protein Ag0932
Full Name peroxisomal biogenesis factor 14
Calculated molecular weight 41 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC006327
Gene symbol PEX14
Gene ID (NCBI) 5195
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Protocols

Product Specific Protocols
IF protocol for CL488 PEX14 antibody CL488-10594Download protocol
Standard Protocols
Click here to view our Standard Protocols