Validation Data Gallery
|Positive WB detected in||human brain tissue, L02 cells, mouse kidney tissue, mouse brain tissue, human kidney tissue, HEK-293 cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12545-1-AP targets PEX5 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PEX5 fusion protein Ag3268|
|Full Name||peroxisomal biogenesis factor 5|
|Calculated molecular weight||631 aa, 70 kDa|
|Observed molecular weight||68-80 kDa|
|GenBank accession number||BC010621|
|Gene ID (NCBI)||5830|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).
|Product Specific Protocols|
|WB protocol for PEX5 antibody 12545-1-AP||Download protocol|
|IHC protocol for PEX5 antibody 12545-1-AP||Download protocol|
|IF protocol for PEX5 antibody 12545-1-AP||Download protocol|
|IP protocol for PEX5 antibody 12545-1-AP||Download protocol|
|FC protocol for PEX5 antibody 12545-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Mol Cell Neurosci
Aging lowers PEX5 levels in cortical neurons in male and female mouse brains.
Eur J Pharmacol
PEX5 prevents cardiomyocyte hypertrophy via suppressing the redox-sensitive signaling pathways MAPKs and STAT3.
Biochim Biophys Acta Mol Cell Biol Lipids
Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.
Hum Mol Genet
A novel type of rhizomelic chondrodysplasia punctata, RCDP5, is caused by loss of the PEX5 long isoform.
Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.
Nat Cell Biol
ATM functions at the peroxisome to induce pexophagy in response to ROS.
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Zhihui (Verified Customer) (12-12-2018)