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  • KD/KO Validated

PEX5 Polyclonal antibody

PEX5 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 12545-1-AP

Synonyms

Peroxin 5, Peroxisome receptor 1, PEX5, PTS1 BP, PTS1 receptor, PTS1R, PXR1



Tested Applications

Positive WB detected inhuman brain tissue, L02 cells, mouse kidney tissue, mouse brain tissue, human kidney tissue, HEK-293 cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12545-1-AP targets PEX5 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PEX5 fusion protein Ag3268
Full Name peroxisomal biogenesis factor 5
Calculated molecular weight 631 aa, 70 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC010621
Gene symbol PEX5
Gene ID (NCBI) 5830
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).

Protocols

Product Specific Protocols
WB protocol for PEX5 antibody 12545-1-APDownload protocol
IHC protocol for PEX5 antibody 12545-1-APDownload protocol
IF protocol for PEX5 antibody 12545-1-APDownload protocol
IP protocol for PEX5 antibody 12545-1-APDownload protocol
FC protocol for PEX5 antibody 12545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Mol Cell Neurosci

Aging lowers PEX5 levels in cortical neurons in male and female mouse brains.

Authors - Ndidi-Ese Uzor
ratWB

Eur J Pharmacol

PEX5 prevents cardiomyocyte hypertrophy via suppressing the redox-sensitive signaling pathways MAPKs and STAT3.

Authors - Minghui Wang

Biochim Biophys Acta Mol Cell Biol Lipids

Ceramide regulates interaction of Hsd17b4 with Pex5 and function of peroxisomes.

Authors - Zhihui Zhu
humanWB

Hum Mol Genet

A novel type of rhizomelic chondrodysplasia punctata, RCDP5, is caused by loss of the PEX5 long isoform.

Authors - Tuva Barøy
humanWB,IF,IP

Autophagy

Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance.

Authors - Michael S Dahabieh
humanWB, IF

Nat Cell Biol

ATM functions at the peroxisome to induce pexophagy in response to ROS.

Authors - Jiangwei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Zhihui (Verified Customer) (12-12-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T And mouse astrocytes
PEX5 Antibody Western Blot, validation (1:1000 dilution) in HEK293T And mouse astrocytes (Cat no:12545-1-AP)