Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, human brain tissue, human kidney tissue, L02 cells, mouse kidney tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | Raji cells, mouse kidney tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12545-1-AP targets PEX5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3268 Product name: Recombinant human PEX5 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 340-639 aa of BC010621 Sequence: EGDLPNAVLLFEAAVQQDPKHMEAWQYLGTTQAENEQELLAISALRRCLELKPDNQTALMALAVSFTNESLQRQACETLRDWLRYTPAYAHLVTPAEEGAGGAGLGPSKRILGSLLSDSLFLEVKELFLAAVRLDPTSIDPDVQCGLGVLFNLSGEYDKAVDCFTAALSVRPNDYLLWNKLGATLANGNQSEEAVAAYRRALELQPGYIRSRYNLGISCINLGAHREAVEHFLEALNMQRKSRGPRGEGGAMSENIWSTLRLALSMLGQSDAYGAADARDLSTLLTMFGLPQ Predict reactive species |
| Full Name | peroxisomal biogenesis factor 5 |
| Calculated Molecular Weight | 631 aa, 70 kDa |
| Observed Molecular Weight | 68-80 kDa |
| GenBank Accession Number | BC010621 |
| Gene Symbol | PEX5 |
| Gene ID (NCBI) | 5830 |
| RRID | AB_2268102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P50542 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The peroxisomal targeting signal type1 (PTS1) receptor, PEX5, is one member of peroxins (PEXs) which are proteins required for peroxisome assembly. PEX5 and PEX7 function as receptors that recognize PTS1- and PTS2- containing proteins, respectively, and PEX5 binds PTS1 through its C-terminal 40-kDa tetratricopeptide repeat domain. It is a predominantly cytoplasmic, partly peroxisomal protein that appears to shuttle between these compartments as it mediates the import of PTS1-containing proteins. PEX5 has been reported to interact with PEX10, PEX12, PEX13, and PEX14. Defects in PEX5 are a cause of Zellweger syndrome (ZWS), which is a lethal peroxisome biogenesis disorder. PEX5 is expressed as two variants through alternative splicing: a shorter variant (PEX5S) that recognizes only the PTS1 cargo protein, and a longer variant (PEX5L) that includes an additional PEX7 binding site(PMID: 17028014; 9668159; 36299769;). This antibody recognizes endogenous PEX5, which migrates with an apparent molecular mass of 80 kDa (PMID: 7790377).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PEX5 antibody 12545-1-AP | Download protocol |
| IF protocol for PEX5 antibody 12545-1-AP | Download protocol |
| IHC protocol for PEX5 antibody 12545-1-AP | Download protocol |
| IP protocol for PEX5 antibody 12545-1-AP | Download protocol |
| WB protocol for PEX5 antibody 12545-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PEX5 (Cat# 12545-1-AP) has 27 citations published in journals including Nature, Nat Cell Biol, Sci Adv, EMBO J, J Clin Invest, Autophagy, PLoS Pathog, J Cell Biol, Front Med, JCI Insight, Cell Mol Life Sci, EMBO Rep, Eur J Pharmacol, J Neurochem, Hum Mol Genet, PLoS One, and others. Research fields span peroxisome biology, pexophagy, lipid metabolism, autophagy, oncology, neurodegeneration, infectious disease, and cardiovascular biology.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol A tuberous sclerosis complex signalling node at the peroxisome regulates mTORC1 and autophagy in response to ROS.
| ||
Autophagy Silencing PEX26 as an unconventional mode to kill drug-resistant cancer cells and forestall drug resistance. | ||
J Biomed Sci PEX11B palmitoylation couples peroxisomal dysfunction with Schwann cells fail in diabetic neuropathy | ||
J Clin Invest Peroxisomal integrity in demyelination-associated microglia enables cellular debris clearance and myelin renewal in mice.
| ||
Sci Adv FAF2 is a bifunctional regulator of peroxisomal homeostasis and saturated lipid responses |
Reviews
What customers say
The product has one review from Zhihui Zhu (December 2018), who rated it 5 stars. The reviewer used the antibody for Western Blot at a 1:1000 dilution on HEK293T cells and mouse astrocytes. No additional written comments were provided.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zhihui (Verified Customer) (12-12-2018) |
 |
