PFAS Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83074-2-RR (same clone as 83074-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83074-2-RR (PFAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83074-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00064-1

  Sandwich ELISA standard curve of MP00064-1, Human PFAS Recombinant Matched Antibody Pair - PBS only. 83074-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20162. 83074-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

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  Sample test of MP00064-1

  HEK-293T cell lysate was measured. The human PFAS concentration of detected samples was determined to be 5.10 ng/mL (based on a 1.4 mg/mL extract load).

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  FC experiment of A549 using 83074-2-RR (same clone as 83074-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.4 ug PFAS Recombinant antibody (83074-2-RR, Clone:230422D11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83074-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00064-1

  Cytometric bead array standard curve of MP00064-1, PFAS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83074-2-PBS. Detection antibody: 83074-1-PBS. Standard: Ag20162. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83074-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83074-2-PBS against Human PFAS was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83074-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83074-2-RR against Human PFAS were performed. The affinity constant is 0.387 nM.

PFAS Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83074-2-PBS

Clone No.230422D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

230422D11, EC:6.3.5.3, FGAM synthase, FGAMS, FGAR amidotransferase

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Product Information

83074-2-PBS targets PFAS as part of a matched antibody pair:

MP00064-1: 83074-2-PBS capture and 83074-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PFAS fusion protein Ag20162
• Full Name: phosphoribosylformylglycinamidine synthase
• Calculated Molecular Weight: 1338 aa, 145 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC167158
• Gene Symbol: PFAS
• Gene ID (NCBI): 5198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15067
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate.