Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28445-1-AP targets PGAM5 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, sheep, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28195 Product name: Recombinant human PGAM5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-255 aa of BC008196 Sequence: MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDGSLEKDRTLTPLGREQAELTGLRLASLGLKFNKIVHSSMTRAIETTDIISRHLPGVCKVSTDLLREGAPIEPDPPVSHWKPEAVQYYEDGARIEAAFRNYIHRADARQEEDSYEIFICHANVIRYIVCSIPPLLSAGDFVVLGS Predict reactive species |
| Full Name | phosphoglycerate mutase family member 5 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 32 kDa |
| GenBank Accession Number | BC008196 |
| Gene Symbol | PGAM5 |
| Gene ID (NCBI) | 192111 |
| RRID | AB_2881143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96HS1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Phosphoglycerate mutase 5 (PGAM5) is a mitochondrial Serine (Ser)/Threonine (Thr) phosphatase normally located in the inner mitochondrial membrane. Upon mitochondrial dysfunction, PGAM5 recruits and dephosphorylates Drp1 at Ser-637, triggers its GTPase activity and promotes mitochondrial fission. PGAM5 regulates mitophagy by stabilizing PINK1 under stress conditions, which recruits E3 ubiquitin ligase PARKIN for degradation of the damaged mitochondria. PGAM5 can be cleaved and released to the cytoplasm through PARKIN, which activates Wnt signaling and induces mitochondrial biogenesis (PMID: 32439975). PGAM5 has 2 isoforms with the molecular mass of 28 and 32 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PGAM5 antibody 28445-1-AP | Download protocol |
| IHC protocol for PGAM5 antibody 28445-1-AP | Download protocol |
| WB protocol for PGAM5 antibody 28445-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PGAM5 antibody (Cat# 28445-1-AP) has 37 citations spanning journals including Nature Communications, Advanced Science, Cell Death & Disease, Journal of Biological Chemistry, Free Radical Biology and Medicine, and ACS Infectious Diseases. Research fields encompass mitochondrial biology, mitophagy, necroptosis, oxeiptosis, hepatocellular carcinoma, pancreatic cancer, neuroinflammation, Parkinson's disease, viral infections, NASH, sepsis, and diabetic nephropathy, with applications primarily in Western blot, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun Erythroid-intrinsic activation of TLR8 impairs erythropoiesis in inherited anemia | ||
Gut Microbes High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis | ||
J Exp Clin Cancer Res GSTK1 suppresses HCC aggravation via L-carnitine metabolism by PGAM5/DRP1 complex-mediated mitochondrial quality control. | ||
Adv Sci (Weinh) Procyanidin Capsules Combat ALF by Restoring Mitochondrial Homeostasis and Inhibiting Necroptosis via the PGAM5/DRP1/PINK1 Pathway. | ||
Adv Sci (Weinh) Targeting Microglial CD49a Inhibits Neuroinflammation and Demonstrates Therapeutic Potential for Parkinson's Disease. | ||
Adv Sci (Weinh) Ponicidin Promotes Hepatocellular Carcinoma Mitochondrial Apoptosis by Stabilizing Keap1-PGAM5 Complex |
Reviews
What customers say
The product received one review with a 4-star rating. The reviewer used it for Western Blot on human samples at a 1:1000 dilution in 1% BSA and reported very clear results. Overall, the feedback highlights reliable performance and strong signal clarity under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Iram (Verified Customer) (07-03-2020) | 1:1000 dilution in 1%BSA giving a very clear results
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