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  • KD/KO Validated

PGM1 Polyclonal antibody

PGM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15161-1-AP

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1



Tested Applications

Positive WB detected inHeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15161-1-AP targets PGM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGM1 fusion protein Ag7269
Full Name phosphoglucomutase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC001756
Gene symbol PGM1
Gene ID (NCBI) 5236
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PGM1 antibody 15161-1-APDownload protocol
IHC protocol for PGM1 antibody 15161-1-APDownload protocol
IF protocol for PGM1 antibody 15161-1-APDownload protocol
IP protocol for PGM1 antibody 15161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cell Metab

Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.

Authors - Marion Curtis
  • KD Validated
humanWB

Brain

C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.

Authors - Scott P Allen
humanWB

J Hazard Mater

Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.

Authors - Ye Zhu
humanWB

Cancer Lett

Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.

Authors - Xintian Chen
humanWB

Front Cell Dev Biol

Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma

Authors - Wenshuo Zhang
humanWB

iScience

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

Authors - Dong-Xue Ding

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer