PGM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PGM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (1)

Cat no : 15161-1-AP

Synonyms

Glucose phosphomutase 1, PGM 1, PGM1, phosphoglucomutase 1

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Validation Data Gallery

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  WB analysis using 15161-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 15161-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15161-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15161-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15161-1-AP (PGM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 15161-1-AP

  IP result of anti-PGM1 (IP:15161-1-AP, 4ug; Detection:15161-1-AP 1:1000) with mouse skin tissue lysate 3200ug.

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  IHC staining of human hepatocirrhosis using 15161-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15161-1-AP (PGM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 15161-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15161-1-AP (PGM1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15161-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15161-1-AP (PGM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15161-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15161-1-AP (PGM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15161-1-AP

  Immunofluorescent analysis of HepG2 cells, using PGM1 antibody 15161-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 15161-1-AP

  Immunofluorescent analysis of HepG2 cells, using PGM1 antibody 15161-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HepG2 using 15161-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PGM1 antibody (15161-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse skin tissue, HEK-293 cells, Jurkat cells, mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human hepatocirrhosis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

15161-1-AP targets PGM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGM1 fusion protein Ag7269
• Full Name: phosphoglucomutase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC001756
• Gene Symbol: PGM1
• Gene ID (NCBI): 5236
• RRID: AB_2161415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Cell Metab; Fibroblasts Mobilize Tumor Cell Glycogen to Promote Proliferation and Metastasis.; Authors - Marion Curtis ; KD Validated
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	WB	J Hazard Mater; Integrative proteomics and metabolomics approach to elucidate metabolic dysfunction induced by silica nanoparticles in hepatocytes.; Authors - Ye Zhu
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
human	WB	iScience; MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.; Authors - Dong-Xue Ding

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

Works very well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer