PGM1 Monoclonal antibody

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  WB analysis using 66105-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66105-1-Ig (PGM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100.

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  IHC staining of human liver cancer using 66105-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66105-1-Ig (PGM1 Antibody) at dilution of 1:100.

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  IF Staining of HepG2 using 66105-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PGM1 antibody (66105-1-Ig, Clone: 2G5A8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

PGM1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66105-1-Ig

Clone No.2G5A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2G5A8, CDG1T, EC:5.4.2.2, GSD14, Phosphoglucomutase-1

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Tested Applications

• Positive WB detected in: HeLa cells, MDA-MB-231 cells, HepG2 cells, pig liver tissue, rabbit liver tissue, rat liver tissue, mouse liver tissue, pig heart tissue, rabbit heart tissue
• Positive IHC detected in: human colon tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66105-1-Ig targets PGM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7294

  Product name: Recombinant human PGM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 213-562 aa of BC001756

  Sequence: SGPNRLKICIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT

• Full Name: phosphoglucomutase 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC001756
• Gene Symbol: PGM1
• Gene ID (NCBI): 5236
• RRID: AB_2881504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36871
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.