Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-66105 targets PGM1 in IF/ICC applications and shows reactivity with human, mouse, rat, rabbit, pig samples.
| Tested Reactivity | human, mouse, rat, rabbit, pig |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7294 Product name: Recombinant human PGM1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 213-562 aa of BC001756 Sequence: SGPNRLKICIDAMHGVVGPYVKKILCEELGAPANSAVNCVPLEDFGGHHPDPNLTYAADLVETMKSGEHDFGAAFDGDGDRNMILGKHGFFVNPSDSVAVIAANIFSIPYFQQTGVRGFARSMPTSGALDRVASATKIALYETPTGWKFFGNLMDASKLSLCGEESFGTGSDHIREKDGLWAVLAWLSILATRKQSVEDILKDHWQKHGRNFFTRYDYEEVEAEGANKMMKDLEALMFDRSFVGKQFSANDKVYTVEKADNFEYSDPVDGSISRNQGLRLIFTDGSRIVFRLSGTGSAGATIRLYIDSYEKDVAKINQDPQVMLAPLISIALKVSQLQERTGRTAPTVIT Predict reactive species |
| Full Name | phosphoglucomutase 1 |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 61 kDa |
| GenBank Accession Number | BC001756 |
| Gene Symbol | PGM1 |
| Gene ID (NCBI) | 5236 |
| RRID | AB_3084206 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36871 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGM1(Phosphoglucomutase-1) is also named as glucose phosphomutase 1 and belongs to the phosphohexose mutase family. It catalyzes the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 is a major isozyme(PMID:8257433). Defects in PGM1 are the cause of glycogen storage disease type 14 (GSD14)(PMID:19625727). It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PGM1 antibody CL488-66105 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
