• Featured Product
  • KD/KO Validated

PGRMC1 Polyclonal antibody

PGRMC1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12990-1-AP

Synonyms

HPR6.6, MPR, PGRMC, PGRMC1



Tested Applications

Positive WB detected inHEK-293 cells, mouse liver tissue, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse ovary tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman cervical cancer tissue, human lung cancer tissue, human ovary tumor tissue, mouse kidney tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12990-1-AP targets PGRMC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PGRMC1 fusion protein Ag3643
Full Name progesterone receptor membrane component 1
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 22-28 kDa
GenBank Accession NumberBC034238
Gene Symbol PGRMC1
Gene ID (NCBI) 10857
RRIDAB_2164342
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Protocols

Product Specific Protocols
WB protocol for PGRMC1 antibody 12990-1-APDownload protocol
IHC protocol for PGRMC1 antibody 12990-1-APDownload protocol
IP protocol for PGRMC1 antibody 12990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EBioMedicine

The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes.

Authors - Uyen B Chu
  • KO Validated
humanIHC

Int J Cancer

Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients.

Authors - Mir Shakeel U R SU
ratWB

Transl Psychiatry

A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.

Authors - H L Cai
ratWB

Front Pharmacol

A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.

Authors - Ting Cao
humanWB

Biol Reprod

Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells.

Authors - Guangfeng Zhao
ratWB

J Cell Physiol

Progesterone maintains the status of granulosa cells and slows follicle development partly through PGRMC1.

Authors - Xiao-Hua Yuan