PGRMC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PGRMC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 12990-1-AP

Synonyms

PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6

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Validation Data Gallery

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  WB analysis of HEK-293 using 12990-1-AP

  WB result of PGRMC1 antibody (12990-1-AP; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.

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  WB analysis using 12990-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12990-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12990-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12990-1-AP (PGRMC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 12990-1-AP

  IP result of anti-PGRMC1 (IP:12990-1-AP, 3ug; Detection:12990-1-AP 1:1000) with MCF-7 cells lysate 1600ug.

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12990-1-AP (PGRMC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12990-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12990-1-AP (PGRMC1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 12990-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using PGRMC1 antibody (12990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse liver tissue, HepG2 cells, MCF-7 cells, mouse kidney tissue, mouse ovary tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human cervical cancer tissue, human lung cancer tissue, human ovary tumor tissue, mouse kidney tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 16 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

12990-1-AP targets PGRMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PGRMC1 fusion protein Ag3643
• Full Name: progesterone receptor membrane component 1
• Calculated Molecular Weight: 195 aa, 22 kDa
• Observed Molecular Weight: 22-28 kDa
• GenBank Accession Number: BC034238
• Gene Symbol: PGRMC1
• Gene ID (NCBI): 10857
• RRID: AB_2164342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

Publications

Species	Application	Title
human	WB	EBioMedicine; The Sigma-2 Receptor and Progesterone Receptor Membrane Component 1 are Different Binding Sites Derived From Independent Genes.; Authors - Uyen B Chu ; KO Validated
human	IHC	Int J Cancer; Elevated progesterone receptor membrane component 1/sigma-2 receptor levels in lung tumors and plasma from lung cancer patients.; Authors - Mir Shakeel U R SU
rat	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
rat	WB	Front Pharmacol; A Potential Mechanism Underlying the Therapeutic Effects of Progesterone and Allopregnanolone on Ketamine-Induced Cognitive Deficits.; Authors - Ting Cao
human	WB	Biol Reprod; Hyaluronic Acid Promotes the Expression of Progesterone Receptor Membrane Component 1 via Epigenetic Silencing of miR-139-5p in Granulosa Cells.; Authors - Guangfeng Zhao
rat	WB	J Cell Physiol; Progesterone maintains the status of granulosa cells and slows follicle development partly through PGRMC1.; Authors - Xiao-Hua Yuan

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