PHEX Monoclonal antibody

PHEX Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Pig and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

1D4B2

Cat no : 66934-1-Ig

Synonyms

HPDR, HPDR1, HYP, HYP1, PHEX, XLH

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Validation Data Gallery

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  WB analysis of HEK-293 using 66934-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 66934-1-Ig

  rat lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig lung using 66934-1-Ig

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66934-1-Ig (PHEX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66934-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66934-1-Ig (PHEX antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat lung tissue, pig lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

66934-1-Ig targets PHEX in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Cited Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PHEX fusion protein Ag20290
• Full Name: phosphate regulating endopeptidase homolog, X-linked
• Calculated Molecular Weight: 749 aa, 86 kDa
• Observed Molecular Weight: 86-100 kDa
• GenBank Accession Number: BC105059
• Gene Symbol: PHEX
• Gene ID (NCBI): 5251
• RRID: AB_2882259
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHEX encodes a transmembrane endopeptidase that belongs to the type II integral membrane zinc-dependent endopeptidase family. The protein usually expressed in osteocytes, osteoblasts and odontoblasts, and it was thought to be involved in bone and dentin mineralization and renal phosphate reabsorption (PMID:28982589). Otherwise, studies showed that PHEX transcripts were identified in human osteosarcoma-derived cells and in differentiated mouse osteoblasts, but not in immature mouse preosteoblasts, indicating the stage-specific expression of the protein (PMID:9199999). Defects in PHEX are responsible for X-linked hypophosphatemic rickets (HYP) (PMID:22339660). Glycosylation of proteins results in higher molecular weight, and about 95-100 kDa band were reported (PMID:25128529; PMID:18728349).

Publications

Species	Application	Title
rat	WB	Acta Biomater; Osteoinductivity and biomechanical assessment of a 3D printed demineralized bone matrix-ceramic composite in a rat spine fusion model.; Authors - Mark A Plantz
human	WB	J Clin Endocrinol Metab; Characterization of novel PHEX variants in X-linked hypophosphatemic rickets and genotype-PHEX activity correlation; Authors - Huixiao Wu