PHF1 Polyclonal antibody

PHF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15663-1-AP

Synonyms

hPCl1, MTF2L2, PCL1, PHD finger protein 1, PHF1, PHF2, Polycomb like protein 1, Protein PHF1

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Validation Data Gallery

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  WB analysis using 15663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15663-1-AP (PHF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15663-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15663-1-AP (PHF1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 15663-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15663-1-AP (PHF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 15663-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15663-1-AP (PHF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 15663-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15663-1-AP (PHF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15663-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15663-1-AP (PHF1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15663-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15663-1-AP (PHF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C2C12 cells, mouse heart tissue, PC-12 cells, mouse liver tissue
• Positive IHC detected in: mouse stomach tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15663-1-AP targets PHF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PHF1 fusion protein Ag8094
• Full Name: PHD finger protein 1
• Calculated Molecular Weight: 567 aa, 62 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC008834
• Gene Symbol: PHF1
• Gene ID (NCBI): 5252
• RRID: AB_2878164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Background

Plant homeodomain finger protein 1 (PHF1) has a specific role in histone modification and in non-homologous end joining. It is a human homolog of the Drosophila protein Polycomb-like protein.

PHF1 acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. PHF1 regulates also p53/TP53 stability.

What is the molecular weight of PHF1?

62 kDa

What is the structure of PHF1?

PHF1 contains two zinc finger-like plant homeodomain (PHD) sequences, a Cys4-His-Cys3 motif that is able to recognize and modify histone tails, making it important in epigenetic regulation (PMID: 18682256). It also contains a Tudor domain, a sequence of approximately 60 amino acids that has also been shown to bind to methylated histone tails (PMID: 16601153). The sequence of PHF1 is similar to the Polycomb family of genes in Drosophila, chromatin remodeling genes that act in embryogenesis.

What is the role of PHF1?

PHF1 has a key role in histone modification. It acts by binding to the histone H3K36me3, a lysine methylation of histone H3 that is a mark for transcriptional activation (PMID: 17189264). PHF1 then recruits Polycomb repressive complex 2 (PRC2) to mark genes for repression. These epigenetic chromatin modifiers are important in cancer development, gene silencing, and stem cell maintenance.

PHF1 is also found to be recruited to double-strand breaks in DNA and plays an important part in the repair in non-homologous end joining (PMID: 18385154), the main DNA repair process in which ends of DNA are ligated without a homologous template. Interacting with proteins involved in DNA damage response, PH1 appears to have a role beyond the polycomb function (PMID: 18385154).

Publications

Species	Application	Title
mouse	WB	Mol Cell; A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.; Authors - Eric Conway
human	WB	Mol Cell; PALI1 promotes tumor growth through competitive recruitment of PRC2 to G9A-target chromatin for dual epigenetic silencing; Authors - Ka-Wing Fong