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  • KD/KO Validated

PHKB Polyclonal antibody

PHKB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13400-1-AP

Synonyms

PHKB, phosphorylase kinase, beta



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, LNCaP cells, mouse heart tissue, rat heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse liver tissue, human skeletal muscle tissue, human liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13400-1-AP targets PHKB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PHKB fusion protein Ag4240
Full Name phosphorylase kinase, beta
Calculated Molecular Weight 1086 aa, 124 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC033657
Gene Symbol PHKB
Gene ID (NCBI) 5257
RRIDAB_2237183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PHKB gene encodes phosphorylase kinase subunit beta involved in glycan biosynthesis and glycogen metabolism. PHKB activity is regulated by phosphorylation of various serine residues, and catalyzes the phosphorylation of serine in certain substrates, including troponin I. Phosphorylase kinase (PhK) complex, composed of alpha, beta, gamma, and delta subunits, stimulates energy production from glycogen in the cascade activation of glycogenolysis. Its large homologous alpha and beta subunits regulate the activity of the catalytic gamma subunit. Defects in PHKB are the cause of glycogen storage disease type 9B (GSD9B) also known as phosphorylase kinase deficiency of liver and muscle (PKD), characterized by hepathomegaly, only slightly elevated transaminases and plasma lipids, clinical improvement with increasing age, and remarkably no clinical muscle involvement.

Protocols

Product Specific Protocols
WB protocol for PHKB antibody 13400-1-APDownload protocol
IHC protocol for PHKB antibody 13400-1-APDownload protocol
IP protocol for PHKB antibody 13400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP, WB

Mol Endocrinol

20-HETE induces hyperglycemia through the cAMP/PKA-PhK-GP pathway.

Authors - Lai Guangrui G
mouseWB

iScience

Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.

Authors - Motoyasu Hosokawa
humanWB,IHC

J Cancer Res Clin Oncol

Phosphorylase kinase β affects colorectal cancer cell growth and represents a novel prognostic biomarker.

Authors - Guanghui Wang
  • KD Validated
pigWB

J Biol Chem

Circular RNA circMYLK4 shifts energy metabolism from glycolysis to OXPHOS by binding to the calcium channel auxiliary subunit CACNA2D2

Authors - Haigang Cao