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PI3 Kinase p85 Alpha Monoclonal antibody

PI3 Kinase p85 Alpha Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4G3C11

Cat no : 60225-1-Ig

Synonyms

GRB1, p85, p85 ALPHA, PI 3 Kinase p85 alpha, PI 3-kinase p85α, PI3 Kinase p85 Alpha, PI3 kinase subunit p85 alpha, PI3K p85(alpha), PI3K regulatory subunit alpha, PIK3R1



Tested Applications

Positive WB detected inA549 cells, rat brain tissue, pig brain tissue, Jurkat cells, HEK-293 cells, fetal human brain tissue, mouse brain tissue, Neuro-2a cells, PC-12 cells
Positive IP detected inRAW 264.7 cells
Positive IHC detected inmouse lung tissue, human prostate cancer tissue, human skeletal muscle tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60225-1-Ig is PI3 Kinase p85 Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PI3 Kinase p85 Alpha fusion protein Ag2344
Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC030815
Gene symbol PIK3R1
Gene ID (NCBI) 5295
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Phosphatidylinositol 3-kinase (PI3K) plays an important role in the metabolic actions of INS and is required for adipogenesis. The PI3K pathway has also been identified as an important player in cancer development and progression. The Class IA PI3K heterodimer, which is composed of a P110 catalytic subunit and a P85 regulatory subunit, is activated upon association of the P85 subunit with upstream adaptor proteins or receptor tyrosine kinases. Mutations in PIK3R1 are implicated in cases of breast cancer and associated to SHORT syndrome.

Protocols

Product Specific Protocols
WB protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IHC protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
IP protocol for PI3 Kinase p85 Alpha antibody 60225-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Pharm Pharmacol

Minimally modified low-density lipoprotein upregulates the ETB and α1 receptors in mouse mesenteric arteries in vivo by activating the PI3K/Akt pathway.

Authors - Zhong-San Zeng
humanWB

Cell Death Differ

Glutathione S-transferases P1 protects breast cancer cell from adriamycin-induced cell death through promoting autophagy.

Authors - Xiaoliang Dong
humanWB

Evid Based Complement Alternat Med

Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

Authors - Yubo Guo
WB

Inflammation

TNF-α Regulates ITGβ1 and SYND4 Expression in Nucleus Pulposus Cells: Activation of FAK/PI3K Signaling.

Authors - Xinghuo Wu
humanWB

Biol Pharm Bull

Picropodophyllin Inhibits the Proliferation of Human Prostate Cancer DU145 and LNCaP Cells via ROS Production and PI3K/AKT Pathway Inhibition.

Authors - Xuejie Zhu
humanWB

Acta Pharmacol Sin

Adiponectin receptor agonist AdipoRon modulates human and mouse platelet function.

Authors - Xiang-Hui Zhou