|Positive WB detected in||HeLa cells, mouse liver tissue, Jurkat cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 23274-1-AP is PINK1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig, rabbit, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PINK1 fusion protein Ag19825|
|Full Name||PTEN induced putative kinase 1|
|Calculated molecular weight||581 aa, 63 kDa|
|Observed molecular weight||65 kDa, 45 kDa|
|GenBank accession number||BC028215|
|Gene ID (NCBI)||65018|
|Purification Method||Antigen Affinity purified|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yahir Alberto (Verified Customer) (11-12-2021)
The antibody works fine with overnight incubation at 4 °C.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.
HONGXUE (Verified Customer) (08-19-2019)
The specific is not good. You can detect many bands using WB.