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  • KD/KO Validated

PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23274-1-AP

Synonyms

BRPK, PARK6, PINK1, PTEN induced putative kinase 1



Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, Jurkat cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23274-1-AP is PINK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PINK1 fusion protein Ag19825
Full Name PTEN induced putative kinase 1
Calculated molecular weight 581 aa, 63 kDa
Observed molecular weight 65 kDa, 45 kDa
GenBank accession numberBC028215
Gene symbol PINK1
Gene ID (NCBI) 65018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa fomrs (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Protocols

Product Specific Protocols
WB protocol for PINK1 antibody 23274-1-APDownload protocol
IHC protocol for PINK1 antibody 23274-1-APDownload protocol
IF protocol for PINK1 antibody 23274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Ecotoxicol Environ Saf

Bone marrow mesenchymal stem cells repair Cr (VI)- injured kidney by regulating mitochondria-mediated apoptosis and mitophagy mediated via the MAPK signaling pathway.

Authors - Fei Yin
mouseWB

Front Endocrinol (Lausanne)

Hepatic DNA Damage Induced by Electronic Cigarette Exposure Is Associated With the Modulation of NAD+/PARP1/SIRT1 Axis.

Authors - Jorge Espinoza-Derout
humanWB

Oxid Med Cell Longev

Bcl-2 Proteins Regulate Mitophagy in Lipopolysaccharide-Induced Acute Lung Injury via PINK1/Parkin Signaling Pathway.

Authors - Zhihao Zhang
mouseIHC

BMC Complement Med Ther

Qiliqiangxin reduced cardiomyocytes apotosis and improved heart function in infarcted heart through Pink1/Parkin -mediated mitochondrial autophagy.

Authors - Junyang Zhou
mouseWB,IF

Cell Death Dis

Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.

Authors - Yuexin Tian
  • KD Validated
WB

Comp Biochem Physiol A Mol Integr Physiol

Novel ultrastructural findings on cardiac mitochondria of huddling Brandt's voles in mild cold environment.

Authors - Zhe Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2 lysate
PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)