PINK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

206

Reviews (4)

Cat no : 23274-1-AP

Synonyms

BRPK, PARK6, PINK1, PTEN induced putative kinase 1

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Validation Data Gallery

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  WB analysis of HeLa using 23274-1-AP

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells.

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  WB analysis of HeLa using 23274-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23274-1-AP

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23274-1-AP (PINK1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, PC-12 cells
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 16 publications below
• WB: See 192 publications below
• IHC: See 19 publications below
• IF: See 34 publications below
• CoIP: See 3 publications below

Product Information

23274-1-AP targets PINK1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit, zebrafish, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PINK1 fusion protein Ag19825
• Full Name: PTEN induced putative kinase 1
• Calculated molecular weight: 581 aa, 63 kDa
• Observed molecular weight: 65 kDa, 45 kDa
• GenBank accession number: BC028215
• Gene symbol: PINK1
• Gene ID (NCBI): 65018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu
human	WB	Nucleic Acids Res; DNA damage invokes mitophagy through a pathway involving Spata18.; Authors - Xiuli Dan
mouse	WB	Autophagy; Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development.; Authors - Rei Unno
mouse	WB, IHC	Autophagy; Autophagy regulates functional differentiation of mammary epithelial cells.; Authors - Jessica Elswood
human	IHC,IF	Autophagy; CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma.; Authors - Jingyue Yao
mouse	WB	Autophagy; Restoration of mitophagy ameliorates cardiomyopathy in Barth syndrome.; Authors - Jun Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nin (Verified Customer) (02-27-2023)

It is okay to probe PINK1 with two bands although there are some weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PINK1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:23274-1-AP)

FH

Yahir Alberto (Verified Customer) (11-12-2021)

The antibody works fine with overnight incubation at 4 °C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T cells

PINK1 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:23274-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 lysate

PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)

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