PINK1 Polyclonal antibody

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  WB analysis of HeLa using 23274-1-AP

  WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells.

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  WB analysis of HeLa using 23274-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 23274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23274-1-AP

  Non-treated and CCCP treated HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP (PINK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 23274-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 23274-1-AP (PINK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of rat brain using 23274-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using PINK1 antibody (23274-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

The PINK1 polyclonal antibody (23274-1-AP) is the highest cited PINK1 antibody in the market with 417 citations.

Cat No. 23274-1-AP

Publications(417)

Reviews (5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Synonyms

BRPK, EC:2.7.11.1, PARK6, PTEN induced putative kinase 1, PTEN-induced putative kinase protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, CCCP treated HeLa cells, PC-12 cells
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 19 publications below
• WB: See 385 publications below
• IHC: See 43 publications below
• IF: See 73 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below

Product Information

23274-1-AP targets PINK1 in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, monkey, zebrafish, goat, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PINK1 fusion protein Ag19825
• Full Name: PTEN induced putative kinase 1
• Calculated Molecular Weight: 581 aa, 63 kDa
• Observed Molecular Weight: 65 kDa, 45 kDa
• GenBank Accession Number: BC028215
• Gene Symbol: PINK1
• Gene ID (NCBI): 65018
• RRID: AB_2879244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9BXM7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Cell Biol; Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells; Authors - Huafeng Zhang
human	WB	Nat Commun; Gonococcal OMV-delivered PorB induces epithelial cell mitophagy; Authors - Shuai Gao
human	WB	Mol Cell; The HRI branch of the integrated stress response selectively triggers mitophagy; Authors - Yogaditya Chakrabarty
mouse,human	WB,IHC	Adv Sci (Weinh); WAC Facilitates Mitophagy-mediated MSC Osteogenesis and New Bone Formation via Protecting PINK1 from Ubiquitination-Dependent Degradation; Authors - Shuai Fan
mouse,human	WB,IP	J Hazard Mater; Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1; Authors - Hao Chen
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

aidan (Verified Customer) (04-10-2024)

Good results. Would try higher dilution next time (1:500) on precast gel. Used handcast gel overnight incubation at 4 degrees. Blocked 1 hr 5% milk

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Muller retinal mouse

PINK1 Antibody Western Blot validation (1:1000 dilution) in Muller retinal mouse (Cat no:23274-1-AP)

FH

Nin (Verified Customer) (02-27-2023)

It is okay to probe PINK1 with two bands although there are some weak non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PINK1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:23274-1-AP)

FH

Yahir Alberto (Verified Customer) (11-12-2021)

The antibody works fine with overnight incubation at 4 °C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK 293T cells

PINK1 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:23274-1-AP)

FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 lysate

PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)