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- KD/KO Validated
PINK1 Polyclonal antibody
PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 23274-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, Jurkat cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 24 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 23274-1-AP is PINK1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, swine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PINK1 fusion protein Ag19825 |
| Full Name | PTEN induced putative kinase 1 |
| Calculated molecular weight | 581 aa, 63 kDa |
| Observed molecular weight | 65 kDa, 45 kDa |
| GenBank accession number | BC028215 |
| Gene symbol | PINK1 |
| Gene ID (NCBI) | 65018 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa fomrs (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PINK1 antibody 23274-1-AP | Download protocol |
| IHC protocol for PINK1 antibody 23274-1-AP | Download protocol |
| IF protocol for PINK1 antibody 23274-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf Bone marrow mesenchymal stem cells repair Cr (VI)- injured kidney by regulating mitochondria-mediated apoptosis and mitophagy mediated via the MAPK signaling pathway. | ||
Front Endocrinol (Lausanne) Hepatic DNA Damage Induced by Electronic Cigarette Exposure Is Associated With the Modulation of NAD+/PARP1/SIRT1 Axis. | ||
Oxid Med Cell Longev Bcl-2 Proteins Regulate Mitophagy in Lipopolysaccharide-Induced Acute Lung Injury via PINK1/Parkin Signaling Pathway. | ||
BMC Complement Med Ther Qiliqiangxin reduced cardiomyocytes apotosis and improved heart function in infarcted heart through Pink1/Parkin -mediated mitochondrial autophagy. | ||
Cell Death Dis Nestin protects podocyte from injury in lupus nephritis by mitophagy and oxidative stress.
| ||
Comp Biochem Physiol A Mol Integr Physiol Novel ultrastructural findings on cardiac mitochondria of huddling Brandt's voles in mild cold environment. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
|
FH HONGXUE (Verified Customer) (08-19-2019) | The specific is not good. You can detect many bands using WB.
 |
