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PINK1 Antibody 18 Publications

Rabbit Polyclonal| Catalog number: 23274-1-AP

Featured Product KD/KO validated

2 customer reviews

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Con: 30 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

PINK1 Antibody WB HeLa cells 23274-1-AP

WB result of PINK1 antibody (23274-1-AP, 1:600) with si-Control and si-PINK1 transfected HeLa cells.

PINK1 Antibody WB HeLa cells 23274-1-AP

HeLa cells were subjected to SDS PAGE followed by western blot with 23274-1-AP( PINK1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

PINK1 Antibody IHC mouse brain tissue 23274-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PINK1 Antibody IHC mouse brain tissue 23274-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23274-1-AP (PINK1 antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PINK1 Antibody IF mouse brain tissue 23274-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed mouse brain tissue using 23274-1-AP(PINK1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, swine
Positive WB detected in:
HeLa cells, mouse liver tissue, Jurkat cells
Positive IHC detected in:
mouse brain tissue, human brain tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse brain tissue
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen Affinity purified
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PINK1 fusion protein Ag19825
Full name:
PTEN induced putative kinase 1
Calculated molecular weight:
581aa,63 kDa
Observed molecular weight:
65 kDa, 45 kDa
GenBank accession number:
BC028215
Gene ID (NCBI):
65018
Gene symbol
PINK1
Synonyms
BRPK, PARK6, PINK1, PTEN induced putative kinase 1

Background

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa fomrs (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

PINK1 Rabbit Polyclonal antibody

Tanusree (Verified Customer), 12-03-2019
Applications
Western Blot,
Primary Antibody Dilution
1:500
No image submitted with review

No image submitted with review

Cell/Tissue Type
Mouse brain
Lot Number
00078625
This antibody works good in western blotting analysis using mouse tissues.

PINK1 Rabbit Polyclonal antibody

HONGXUE (Verified Customer), 08-19-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)

PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)

Cell/Tissue Type
HepG2 lysate
Lot Number
00061565
The specific is not good. You can detect many bands using WB.
Proteintech Response
Thank you for your review. One possibility is that you are seeing multiple isoforms of this protein: 63-66 kDa (full length) , 52-55 kDa (cleaved PINK1-S), 46 kDa (processed form) ,30 kDa(isoform, https://www.uniprot.org/uniprot/Q9BXM7). We will contact you to see how we can support you to achieve high quality results.

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