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  • KD/KO Validated

PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23274-1-AP

Synonyms

BRPK, PARK6, PINK1, PTEN induced putative kinase 1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23274-1-AP is PINK1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PINK1 fusion protein Ag19825
Full Name PTEN induced putative kinase 1
Calculated molecular weight 581 aa, 63 kDa
Observed molecular weight 65 kDa, 45 kDa
GenBank accession numberBC028215
Gene symbol PINK1
Gene ID (NCBI) 65018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Protocols

Product Specific Protocols
WB protocol for PINK1 antibody 23274-1-APDownload protocol
IHC protocol for PINK1 antibody 23274-1-APDownload protocol
IF protocol for PINK1 antibody 23274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu
humanWB

Nucleic Acids Res

DNA damage invokes mitophagy through a pathway involving Spata18.

Authors - Xiuli Dan
mouseWB

Autophagy

Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development.

Authors - Rei Unno
mouseWB, IHC

Autophagy

Autophagy regulates functional differentiation of mammary epithelial cells.

Authors - Jessica Elswood
humanIHC,IF

Autophagy

CDK9 inhibition blocks the initiation of PINK1-PRKN-mediated mitophagy by regulating the SIRT1-FOXO3-BNIP3 axis and enhances the therapeutic effects involving mitochondrial dysfunction in hepatocellular carcinoma.

Authors - Jingyue Yao
mouseWB

Autophagy

Restoration of mitophagy ameliorates cardiomyopathy in Barth syndrome.

Authors - Jun Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yahir Alberto (Verified Customer) (11-12-2021)

The antibody works fine with overnight incubation at 4 °C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T cells
PINK1 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:23274-1-AP)
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2 lysate
PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)