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  • KD/KO Validated

PINK1 Polyclonal antibody

PINK1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 23274-1-AP

Synonyms

BRPK, PARK6, PINK1, PTEN induced putative kinase 1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, PC-12 cells
Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23274-1-AP targets PINK1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, monkey, rabbit, zebrafish, pig, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PINK1 fusion protein Ag19825
Full Name PTEN induced putative kinase 1
Calculated Molecular Weight 581 aa, 63 kDa
Observed Molecular Weight 65 kDa, 45 kDa
GenBank Accession NumberBC028215
Gene Symbol PINK1
Gene ID (NCBI) 65018
RRIDAB_2879244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PINK1 is a mitochondrial serine/threonine-protein kinase that protects cells from stress-induced mitochondrial dysfunction. The precursor of PINK1 (65 kDa) is synthesized in the cytosol and is imported into the outer membrane of mitochondria. PINK1 is further transferred into the inner membrane. The full-length PINK1 can be proteolytically processed into 52-55 kDa and 45-46 kDa forms (PMID: 18221368; 25108683; 18031932). The half-life of the mature form of PINK1 is very short and it was proposed that the proteasome is involved in its degradation (PMID: 23472196). The gene of PINK1 maps to chromosome 1p36.12. Two alternatively spliced variants exist, the shorter isoform (30 kDa) produced by alternative splicing. Mutations in the PINK1 gene cause autosomal recessive early-onset Parkinson's disease.

Protocols

Product Specific Protocols
WB protocol for PINK1 antibody 23274-1-APDownload protocol
IHC protocol for PINK1 antibody 23274-1-APDownload protocol
IF protocol for PINK1 antibody 23274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Gonococcal OMV-delivered PorB induces epithelial cell mitophagy

Authors - Shuai Gao
humanWB

Mol Cell

The HRI branch of the integrated stress response selectively triggers mitophagy

Authors - Yogaditya Chakrabarty
human,mouseWB,IP

J Hazard Mater

Low-dose deoxynivalenol exposure inhibits hepatic mitophagy and hesperidin reverses this phenomenon by activating SIRT1

Authors - Hao Chen
mouseWB

Nat Commun

Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.

Authors - Zhisheng Xu
humanWB

Nucleic Acids Res

DNA damage invokes mitophagy through a pathway involving Spata18.

Authors - Xiuli Dan
mouseWB

Autophagy

Deregulated MTOR (mechanistic target of rapamycin kinase) is responsible for autophagy defects exacerbating kidney stone development.

Authors - Rei Unno

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

aidan (Verified Customer) (04-10-2024)

Good results. Would try higher dilution next time (1:500) on precast gel. Used handcast gel overnight incubation at 4 degrees. Blocked 1 hr 5% milk

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Muller retinal mouse
PINK1 Antibody Western Blot validation (1:1000 dilution) in Muller retinal mouse (Cat no:23274-1-AP)
FH

Nin (Verified Customer) (02-27-2023)

It is okay to probe PINK1 with two bands although there are some weak non-specific bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PINK1 Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:23274-1-AP)
FH

Yahir Alberto (Verified Customer) (11-12-2021)

The antibody works fine with overnight incubation at 4 °C.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T cells
PINK1 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:23274-1-AP)
FH

Tanusree (Verified Customer) (12-03-2019)

This antibody works good in western blotting analysis using mouse tissues.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

HONGXUE (Verified Customer) (08-19-2019)

The specific is not good. You can detect many bands using WB.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HepG2 lysate
PINK1 Antibody Western Blot, validation (1:1000 dilution) in HepG2 lysate (Cat no:23274-1-AP)