GCDFP-15/PIP Polyclonal antibody

GCDFP-15/PIP Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC,ELISA

Conjugate

Unconjugated

Cat no : 16068-1-AP

Synonyms

GCDFP 15, GCDFP15, gp17, GPIP4, PIP, prolactin induced protein, Prolactin inducible protein, SABP

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Validation Data Gallery

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 16068-1-AP (GCDFP-15/PIP antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16068-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 16068-1-AP (GCDFP-15/PIP antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human skin tissue, human breast tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16068-1-AP targets GCDFP-15/PIP in IHC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GCDFP-15/PIP fusion protein Ag9004
• Full Name: prolactin-induced protein
• Calculated Molecular Weight: 146 aa, 17 kDa
• GenBank Accession Number: BC010950
• Gene Symbol: PIP
• Gene ID (NCBI): 5304
• RRID: AB_2878212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCDFP-15 (gross cystic disease fluid protein 15), also known as PIP (prolactin-induced protein) is a secretory glycoprotein expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands (PMID: 12393800). GCDFP-15 expression is increased by prolactin and androgen and inhibited by estrogen (PMID: 18854942). The expression is also regulated by interleukins. GCDFP-15 is a marker of apocrine differentiation and is frequently used for assessment of metastases or regional recurrences of breast origin.