PIP5K1A Polyclonal antibody

• Featured Product
• KD/KO Validated

PIP5K1A Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

12

Reviews (1)

Cat no : 15713-1-AP

Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha

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Validation Data Gallery

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  WB analysis using 15713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 15713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15713-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15713-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15713-1-AP (PIP5K1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 15713-1-AP

  IP result of anti-PIP5K1A (15713-1-AP for IP and Detection).

• 

  IP experiment of mouse heart using 15713-1-AP

  IP result of anti-PIP5K1A (IP:15713-1-AP, 4ug; Detection:15713-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human tonsillitis using 15713-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15713-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15713-1-AP (PIP5K1A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15713-1-AP

  Immunofluorescent analysis of Hela cells, using PIP5K1A antibody 15713-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 15713-1-AP

  1X10^6 HeLa cells were stained with .5ug PIP5K1A antibody (15713-1-AP, red) and control antibody (blue). FITC-Goat anti-Rabbit IgG with dilution 1:100. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, human liver tissue, human heart tissue, NCI-H1299 cells, PC-12 cells
• Positive IP detected in: HeLa cells, mouse heart tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 5 publications below
• IP: See 2 publications below

Product Information

15713-1-AP targets PIP5K1A in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIP5K1A fusion protein Ag8340
• Full Name: phosphatidylinositol-4-phosphate 5-kinase, type I, alpha
• Calculated Molecular Weight: 56 kDa, 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC007833
• Gene Symbol: PIP5K1A
• Gene ID (NCBI): 8394
• RRID: AB_2164696
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly(PMID:17688436). There are three PIP5K isoforms,α, β, and γ. The nomenclature for the α and β isoforms is switched between humans and mice(PMID:22096541). There are also several splicing variants of the γ isozyme have been identified.(PMID:20945365). PIP5K1A has some isoforms produced by alternative splicing with the molecular mass of 56-63 kDa.

Publications

Species	Application	Title
rat	WB, IF	Nat Cell Biol; Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation.; Authors - Rong Yueguang Y
human	WB	J Clin Invest; Spastic paraplegia proteins spastizin and spatacsin mediate autophagic lysosome reformation.; Authors - Jaerak Chang
human	WB	EMBO J; Phosphatidylinositol 4,5-bisphosphate controls Rab7 and PLEKHM1 membrane cycling during autophagosome-lysosome fusion.; Authors - Takashi Baba
human	WB,IF,IP,IHC	Oncogene; The role of PIP5K1α/pAKT and targeted inhibition of growth of subtypes of breast cancer using PIP5K1α inhibitor.; Authors - Martuza Sarwar ; KD Validated
mouse	WB	Nutrients; Effects of Gestational Diabetes Mellitus and Selenium Deficiency on the Offspring Growth and Blood Glucose Mechanisms of C57BL/6J Mice; Authors - Wenhui Xu
human	WB,IF	Front Cell Dev Biol; PIP5K1α is Required for Promoting Tumor Progression in Castration-Resistant Prostate Cancer.; Authors - Tianyan Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bastien (Verified Customer) (08-19-2020)

Staining of B cells from mice bone marrow after cytoplasmic permezbiliation. The cells were first fixed and permeabilized with intracellular fix and perm set from ebioscience and then stained with 0,25 µl/ million of cells during 50min After, a seconde staining was performed with 0,1µl/million cells of F(ab')2-Donkey anti-Rabbit IgG (H+L), PE, Secondary Antibody from invitrogen. In red secondary antibody alone and in blue primary + secondary antibody

• Applications: Flow Cytometry
• Primary Antibody Dilution: 0,25µl/10^6cells
• Cell Tissue Type: B cells

PIP5K1A Antibody Flow Cytometry validation (0,25µl/10^6cells dilution) in B cells (Cat no:15713-1-AP)