PJA1 Polyclonal antibody

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  WB analysis using 17687-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17687-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17687-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17687-1-AP (PJA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17687-1-AP

  IP result of anti-PJA1 (IP:17687-1-AP, 3ug; Detection:17687-1-AP 1:300) with mouse brain tissue lysate 3600ug.

• Featured Product
• KD/KO Validated

PJA1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 17687-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IP, CoIP, ELISA

Synonyms

E3 ubiquitin-protein ligase Praja-1, EC:2.3.2.27, praja ring finger 1, Praja1, RING finger protein 70

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

17687-1-AP targets PJA1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11820

  Product name: Recombinant human PJA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 294-643 aa of BC075803

  Sequence: ANDNEGHSDGLARRGRGESSSGYPEPKYPEDKREARSDQVKPEKVPRRRRTMADPDFWTHSDDYYKYCDEDSDSDKEWIAALRRKYRSREQTLSSSGESWETLPGKEEREPPQAKVSASTGTSPGPGASASAGAGAGASAGSNGSNYLEEVREPSLQEEQASLEEGEIPWLQYHENDSSSEGDNDSGHELMQPGVFMLDGNNNLEDDSSVSEDLEVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDALPEILVTEDHGAVGQEMCCPICCSEYVKGEVATELPCHHYFHKPCVSIWLQKSGTCPVCRCMFPPPL

• Full Name: praja ring finger 1
• Calculated Molecular Weight: 643 aa, 71 kDa
• Observed Molecular Weight: 71-80 kDa
• GenBank Accession Number: BC075803
• Gene Symbol: PJA1
• Gene ID (NCBI): 64219
• RRID: AB_2252483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NG27
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Nat Commun; Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.; Authors - Silvia Consalvi ; KD Validated
human	WB	Ann Clin Transl Neurol; A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders.; Authors - Toshimitsu Suzuki
mouse	WB	Biochim Biophys Acta Mol Cell Biol Lipids; Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.; Authors - Qiang Lu
human	WB	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe
rat	CoIP	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.; Authors - Kazuhiko Watabe