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  • KD/KO Validated

PJA1 Polyclonal antibody

PJA1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17687-1-AP

Synonyms

PJA1, praja ring finger 1, Praja1, RING finger protein 70, RNF70



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17687-1-AP targets PJA1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PJA1 fusion protein Ag11820
Full Name praja ring finger 1
Calculated Molecular Weight 643 aa, 71 kDa
Observed Molecular Weight 71-80 kDa
GenBank Accession NumberBC075803
Gene Symbol PJA1
Gene ID (NCBI) 64219
RRIDAB_2252483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.

Protocols

Product Specific Protocols
WB protocol for PJA1 antibody 17687-1-APDownload protocol
IP protocol for PJA1 antibody 17687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.

Authors - Silvia Consalvi
  • KD Validated
humanWB

Ann Clin Transl Neurol

A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders.

Authors - Toshimitsu Suzuki
mouseWB

Biochim Biophys Acta Mol Cell Biol Lipids

Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system.

Authors - Qiang Lu
humanWB

J Virol

PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.

Authors - Wei Xu
ratWB

Neuropathology

Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.

Authors - Kazuhiko Watabe
ratCoIP

Neuropathology

Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation.

Authors - Kazuhiko Watabe