PKC Gamma Polyclonal antibody, PBS Only

• 

  WB analysis using 29208-1-AP (same clone as 29208-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29208-1-AP (PKC Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

PKC Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 29208-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

29208-1-PBS targets PKC Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25085

  Product name: Recombinant human PRKCG protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 600-641 aa of BC047876

  Sequence: SGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGE

• Full Name: protein kinase C, gamma
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC047876
• Gene Symbol: PKC Gamma
• Gene ID (NCBI): 5582
• RRID: AB_2918249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05129
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.