PKM Polyclonal antibody, PBS Only

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  WB analysis of KEK293 cells using 25659-1-AP (same clone as 25659-1-PBS)

  WB result of PKM antibody (25659-1-AP, 1:3000) with si-control and si-PKM transfected HEK293 cell. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis using 25659-1-AP (same clone as 25659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 25659-1-AP (same clone as 25659-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 25659-1-AP (same clone as 25659-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 25659-1-AP (same clone as 25659-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  WB analysis of SMMC-7721 using 25659-1-AP (same clone as 25659-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 25659-1-AP (PKM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 25659-1-AP (same clone as 25659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25659-1-AP (PKM Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25659-1-AP (same clone as 25659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25659-1-AP (PKM antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (25659-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 25659-1-AP (same clone as 25659-1-PBS)

  Immunofluorescent analysis of HeLa cells using 25659-1-AP (PKM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25659-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PKM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 25659-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

25659-1-PBS targets PKM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20270

  Product name: Recombinant human PKM protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-531 aa of BC000481

  Sequence: MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMIKSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP

• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC000481
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• RRID: AB_2880181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P14618
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PKM, also named as OIP3, PK2, PK3, PK-M1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2.(PMID:18337823). This antibody can recognize both PK-M1 and PK-M2.