PLAA Polyclonal antibody, PBS Only

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  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  WB result of PLAA antibody (12529-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLAA transfected MCF-7 cells. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12529-1-AP (same clone as 12529-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12529-1-AP (PLAA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 12529-1-AP (same clone as 12529-1-PBS)

  IP result of anti-PLAA (IP:12529-1-AP, 4ug; Detection:12529-1-AP 1:600) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12529-1-AP (same clone as 12529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12529-1-AP (PLAA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12529-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PLAA Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 12529-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

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Product Information

12529-1-PBS targets PLAA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3224

  Product name: Recombinant human PLAA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 496-795 aa of BC032551

  Sequence: GAGRYVPGSASMGTTMAGVDPFTGNSAYRSAASKTMNIYFPKKEAVTFDQANPTQILGKLKELNGTAPEEKKLTEDDLILLEKILSLICNSSSEKPTVQQLQILWKAINCPEDIVFPALDILRLSIKHPSVNENFCNEKEGAQFSSHLINLLNPKGKPANQLLALRTFCNCFVGQAGQKLMMSQRESLMSHAIELKSGSNKNIHIALATLALNYSVCFHKDHNIEGKAQCLSLISTILEVVQDLEATFRLLVALGTLISDDSNAVQLAKSLGVDSQIKKYSSVSEPAKVSECCRFILNLL

• Full Name: phospholipase A2-activating protein
• Calculated Molecular Weight: 738 aa, 81 kDa
• Observed Molecular Weight: 72-74 kDa
• GenBank Accession Number: BC032551
• Gene Symbol: PLAA
• Gene ID (NCBI): 9373
• RRID: AB_2164881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y263
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Phospholipase A-2-activating protein (PLAA), also named PLAP, can interact with ubiquitin and is involved in the maintenance of ubiquitin levels. PLAA contains multiple consensus domains including WD repeats, PFU domain and PUL domain. Although the function of PLAA remains unclear, ubiquitin binding of PLAA might be the central role that connects ubiquitination and degradation in ERAD. Recent finding revealed that PLAA served as a novel nociveptive mediator after incision, and its expression level is regulated by miR-203.