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uPA/Urokinase Polyclonal antibody
uPA/Urokinase Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 17968-1-AP
Validation Data Gallery
|Positive WB detected in||DU 145 cells, human brain tissue, A375 cells, Hela cells, LNCaP cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human lung tissue, human ovary tissue, human prostate cancer tissue, human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17968-1-AP targets uPA/Urokinase in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||uPA/Urokinase fusion protein Ag11813|
|Full Name||plasminogen activator, urokinase|
|Calculated molecular weight||431 aa, 49 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC013575|
|Gene ID (NCBI)||5328|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).
|Product Specific Protocols|
|WB protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IHC protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IF protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IP protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Aging (Albany NY)
NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.
Macrophages are requisite for angiogenesis of type H vessels during bone regeneration in mice.
E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects.
Urokinase-type Plasminogen Activator Is a Therapeutic Target for Overcoming Sorafenib Resistance in Hepatoma Cells.