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uPA/Urokinase Polyclonal antibody

uPA/Urokinase Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17968-1-AP

Synonyms

ATF, PLAU, U plasminogen activator, uPA, uPA/Urokinase, URK



Tested Applications

Positive WB detected inDU 145 cells, human brain tissue, A375 cells, Hela cells, LNCaP cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human lung tissue, human ovary tissue, human prostate cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17968-1-AP targets uPA/Urokinase in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen uPA/Urokinase fusion protein Ag11813
Full Name plasminogen activator, urokinase
Calculated molecular weight 431 aa, 49 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC013575
Gene symbol PLAU
Gene ID (NCBI) 5328
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing. Defects in PLAU are the cause of Quebec platelet disorder (QPD).

Protocols

Product Specific Protocols
WB protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IHC protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IF protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
IP protocol for uPA/Urokinase antibody 17968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Rg3 inhibits gemcitabine-induced lung cancer cell invasiveness through ROS-dependent, NF-κB- and HIF-1α-mediated downregulation of PTX3.

Authors - Bulbul Ahmmed
humanWB

Oncotarget

Adipocyte-derived exosomes promote lung cancer metastasis by increasing MMP9 activity via transferring MMP3 to lung cancer cells.

Authors - Jiaoli Wang
humanWB

Aging (Albany NY)

NFAT5 directs hyperosmotic stress-induced fibrin deposition and macrophage infiltration via PAI-1 in endothelium.

Authors - Pingping Ma
mouse

Bone

Macrophages are requisite for angiogenesis of type H vessels during bone regeneration in mice.

Authors - Yukihiro Kohara
humanWB

Biochem Cell Biol

MicroRNA-378-3p/5p suppresses migration and invasion of oral squamous carcinoma cells by inhibiting KLK4 expression.

Authors - Zhi Cui
humanWB

Oncol Rep

Identification of potential targets for diallyl disulfide in human gastric cancer MGC-803 cells using proteomics approaches.

Authors - Bo Su