|Positive WB detected in||HeLa cells, A375 cells, human brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human skin tissue, human lung tissue, human ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17968-1-AP targets uPA/Urokinase in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||uPA/Urokinase fusion protein Ag11813|
|Full Name||plasminogen activator, urokinase|
|Calculated molecular weight||431 aa, 49 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC013575|
|Gene ID (NCBI)||5328|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PLAU(Urokinase-type plasminogen activator) is also named as uPA and belongs to the peptidase S1 family.UPA initiates a proteolytic cascade, which degrades extracellular matrix during tissue growth and remodelling.The secreted proform of UPA is activated after binding to a specific high affinity cell surface receptor. Secreted UPA is exposed to rapid inactivation in the pericellular space by plasminogen activator inhibitor-1 (PAI-1), which is stored in the extracellular matrix bound to vitronectin(PMID:9665342).It has 2 isoforms produced by alternative splicing.Defects in PLAU are the cause of Quebec platelet disorder (QPD).
|Product Specific Protocols|
|WB protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IHC protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IF protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|IP protocol for uPA/Urokinase antibody 17968-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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