PLCD3 Polyclonal antibody, PBS Only

• 

  WB analysis of SH-SY5Y using 16792-1-AP (same clone as 16792-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

• 

  WB analysis of U2OS using 16792-1-AP (same clone as 16792-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16792-1-AP (same clone as 16792-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP (same clone as 16792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP (same clone as 16792-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16792-1-PBS in a different storage buffer formulation.

PLCD3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16792-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Phospholipase C-delta-3, phospholipase C, delta 3, Phospholipase C delta 3, Phosphoinositide phospholipase C-delta-3, EC:3.1.4.11

---

Product Information

16792-1-PBS targets PLCD3 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCD3 fusion protein Ag10249
• Full Name: phospholipase C, delta 3
• Calculated Molecular Weight: 789 aa, 89 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC072384
• Gene Symbol: PLCD3
• Gene ID (NCBI): 113026
• RRID: AB_2878316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3E9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.