PLCD3 Polyclonal antibody

PLCD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16792-1-AP

Synonyms

Phospholipase C-delta-3, phospholipase C, delta 3, Phospholipase C delta 3, Phosphoinositide phospholipase C-delta-3, EC:3.1.4.11

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 16792-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 16792-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 16792-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 16792-1-AP (PLCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 16792-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 16792-1-AP (PLCD3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A549 cells, U2OS cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

16792-1-AP targets PLCD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLCD3 fusion protein Ag10249
• Full Name: phospholipase C, delta 3
• Calculated Molecular Weight: 789 aa, 89 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC072384
• Gene Symbol: PLCD3
• Gene ID (NCBI): 113026
• RRID: AB_2878316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phospholipase C Delta 3 (PLCD3) is a protein-coding gene located on chromosome 17 and a member of the phospholipase C family. PLCD3 is expressed broadly across different tissues, with high expression levels observed in the colon and urinary bladder, among others. The enzyme localizes to the plasma membrane and requires calcium for activation. Spermine, sphingosine, and several phospholipids can inhibit its activity. Recent studies have implicated PLCD3 in various types of cancer. In summary, PLCD3 is an important enzyme involved in cellular signaling and has been implicated in the development and progression of various cancers, making it a potential target for therapeutic interventions.

Publications

Species	Application	Title
pig	WB	J Cell Biochem; Phospholipase C inhibits apoptosis of porcine oocytes cultured in vitro.; Authors - Hua Li Chen
human	WB	Oncol Rep; PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells.; Authors - Weidong Liu