Perilipin 1 Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00840-2

  Sandwich ELISA standard curve of MP00840-2, Human Perilipin 1 Recombinant Matched Antibody Pair - PBS only. 83905-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26426. 83905-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00840-2

  Mouse liver tissue lysate was measured. The human Perilipin 1 concentration in mouse liver tissue lysate was determined to be 23.24 ng/mL (based on a 3.70 mg/mL extract load). 3T3-L1 cell lysate was measured. The human PLIN1 concentration in 3T3-L1 cell lysate was determined to be 3.35 ng/mL (based on a 3.40 mg/mL extract load).

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  Cytometric bead array standard curve of MP00840-2

  Cytometric bead array standard curve of MP00840-2, Perilipin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83905-3-PBS. Detection antibody: 83905-1-PBS. Standard: Ag26426. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83905-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 83905-3-PBS against Human Perilipin 1 was performed. The affinity constant is 1.01 nM.

Perilipin 1 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83905-3-PBS

Clone No.240994H8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

PLIN, PERI, PLIN1

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Product Information

83905-3-PBS targets Perilipin 1 as part of a matched antibody pair:

MP00840-2: 83905-3-PBS capture and 83905-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26426

  Product name: Recombinant human PLIN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 91-200 aa of NM_001145311

  Sequence: MACRGLDHLEEKIPALQYPPEKIASELKDTISTRLRSARNSISVPIASTSDKVLGAALAGCELAWGVARDTAEFAANTRAGRLASGGADLALGSIEKVVEYLLPPDKEESA

• Full Name: perilipin
• Calculated Molecular Weight: 56 kDa
• GenBank Accession Number: NM_001145311
• Gene Symbol: Perilipin 1
• Gene ID (NCBI): 5346
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60240
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.