PLK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLK1 Polyclonal Antibody for FC (Intra), IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 10305-1-AP

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13

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Validation Data Gallery

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  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10305-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 10305-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HeLa using 10305-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, HEK-293 cells, A549 cells, HeLa cells, U2OS cells, mouse testis tissue, rat testis tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10305-1-AP targets PLK1 in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK1 fusion protein Ag0284
• Full Name: polo-like kinase 1 (Drosophila)
• Calculated molecular weight: 68 kDa
• Observed molecular weight: 62 kDa
• GenBank accession number: BC002369
• Gene symbol: PLK1
• Gene ID (NCBI): 5347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.; Authors - Wengui Shi
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
	WB	Elife; 53BP1 and BRCA1 control pathway choice for stalled replication restart.; Authors - Yixi Xu
human	WB,IP	Blood Adv; USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis; Authors - Shuguang Liu
human	IHC	Int J Cancer; Impact of iASPP on chemoresistance through PLK1 and autophagy in ovarian clear cell carcinoma.; Authors - Ka-Kui Chan
mouse		Development; PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis.; Authors - Xiukun Wang

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