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- KD/KO Validated
PLK1 Polyclonal antibody
PLK1 Polyclonal Antibody for FC (Intra), IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 10305-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MDA-MB-453s cells, HEK-293 cells, A549 cells, HeLa cells, U2OS cells, mouse testis tissue, rat testis tissue |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 14 publications below |
IHC | See 4 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
10305-1-AP targets PLK1 in WB, IP, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PLK1 fusion protein Ag0284 |
Full Name | polo-like kinase 1 (Drosophila) |
Calculated molecular weight | 68 kDa |
Observed molecular weight | 62 kDa |
GenBank accession number | BC002369 |
Gene symbol | PLK1 |
Gene ID (NCBI) | 5347 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).
Protocols
Product Specific Protocols | |
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WB protocol for PLK1 antibody 10305-1-AP | Download protocol |
IHC protocol for PLK1 antibody 10305-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer. | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
Blood Adv USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis | ||
Int J Cancer Impact of iASPP on chemoresistance through PLK1 and autophagy in ovarian clear cell carcinoma. | ||
Development PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis. |