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PLK1 Polyclonal antibody

PLK1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10305-1-AP

Synonyms

PLK, PLK 1, PLK1, Polo like kinase 1, STPK13



Tested Applications

Positive WB detected inMDA-MB-453s cells, HEK-293 cells, A549 cells, HeLa cells, U2OS cells, mouse testis tissue, rat testis tissue
Positive IHC detected inrat stomach tissue, mouse stomach tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10305-1-AP targets PLK1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK1 fusion protein Ag0284
Full Name polo-like kinase 1 (Drosophila)
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC002369
Gene symbol PLK1
Gene ID (NCBI) 5347
RRIDAB_2877731
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK1(Polo-like kinase 1), also named as PLK and STPK13, belongs to the protein kinase superfamily, Ser/Thr protein kinase family and CDC5/Polo subfamily. PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.The localization of Polo changes during the cell cycle. In interphase, Polo shows a mostly diffuse cytoplasmic localization. Polo becomes concentrated on centrosomes from early prophase and appears on centromeres or kinetochores from late prophase, before nuclear envelope breakdown (NEB). After anaphase onset, Polo relocalizes to the central spindle and remains enriched at the midbody ring in late stages of cytokinesis (PMID: 29167465).

Protocols

Product Specific Protocols
WB protocol for PLK1 antibody 10305-1-APDownload protocol
IHC protocol for PLK1 antibody 10305-1-APDownload protocol
FC protocol for PLK1 antibody 10305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Clin Invest

PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease models

Authors - Marta Baldrighi
humanWB

Adv Sci (Weinh)

Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer

Authors - Huaiyuan Liang
  • KD Validated
human,mouseWB

Sci Adv

Cascade dynamic assembly/disassembly of DNA nanoframework enabling the controlled delivery of CRISPR-Cas9 system

Authors - Nachuan Song
humanWB,IF

Nat Commun

Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer.

Authors - Wengui Shi
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
ratWB

Cell Rep

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

Authors - Kellen D Winden