CoraLite® Plus 488-conjugated PLK1-phospho Recombinant antibody

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  IF Staining of HeLa using CL488-83260-5

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PLK1-phospho antibody (CL488-83260-5, Clone: 240092B10 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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  IF Staining of HepG2 using CL488-83260-5

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PLK1-phospho antibody (CL488-83260-5, Clone: 240092B10 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

PLK1-phospho Recombinant Antibody for IF/ICC

Cat No. CL488-83260-5

Clone No.240092B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

PLK1, 240092B10, EC:2.7.11.21, PLK, PLK 1

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83260-5 targets PLK1-phospho in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: polo-like kinase 1 (Drosophila)
• Calculated Molecular Weight: 68 kDa
• GenBank Accession Number: NM_005030
• Gene Symbol: PLK1
• Gene ID (NCBI): 5347
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P53350
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.