PLK4 Polyclonal antibody

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  WB analysis of A549 cells using 12952-1-AP

  WB result of PLK4 antibody (12952-1-AP, 1:2000) with si-control and si-PLK4 transfected A549 cells.

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  WB analysis using 12952-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12952-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12952-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12952-1-AP (PLK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12952-1-AP (PLK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 12952-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12952-1-AP (PLK4 antibody) at dilution of 1:50 (under 40x lens).

• Featured Product
• KD/KO Validated

PLK4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12952-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

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Tested Applications

• Positive WB detected in: mouse lung tissue, MCF-7 cells, A549 cells
• Positive IHC detected in: mouse testis tissue, human lung cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 11 publications below
• IF: See 6 publications below

Product Information

12952-1-AP targets PLK4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3605

  Product name: Recombinant human PLK4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-263 aa of BC036023

  Sequence: MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLSNLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPPFDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHP

• Full Name: polo-like kinase 4 (Drosophila)
• Calculated Molecular Weight: 970 aa, 109 kDa
• Observed Molecular Weight: 105-112 kDa
• GenBank Accession Number: BC036023
• Gene Symbol: PLK4
• Gene ID (NCBI): 10733
• RRID: AB_2284150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O00444
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse, human	WB, IHC	Nat Commun; Loss of KLF14 triggers centrosome amplification and tumorigenesis.; Authors - Guangjian Fan ; KO Validated
human	IF	Cancer Res; Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.; Authors - Bingteng Xie
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human	WB,IHC	Cell Death Dis; Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.; Authors - Xiangdong Tian ; KD Validated
human	WB	Cell Death Dis; NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.; Authors - Min Li
human	WB	Mol Cancer Ther; Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.; Authors - Ke Liu