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PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12952-1-AP


PLK 4, PLK4, Polo like kinase 4, SAK, STK18

Tested Applications

Positive WB detected inmouse lung tissue, A549 cells, MCF-7 cells
Positive IHC detected inhuman testis tissue, human cervical cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:25-1:100
Sample-dependent, check data in validation data gallery

Product Information

12952-1-AP targets PLK4 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK4 fusion protein Ag3605
Full Name polo-like kinase 4 (Drosophila)
Calculated molecular weight 970 aa, 109 kDa
Observed molecular weight 105-112 kDa
GenBank accession numberBC036023
Gene symbol PLK4
Gene ID (NCBI) 10733
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PLK4(Polo-like kinase 4) is also named as SAK, STK18 and belongs to the Ser/Thr protein kinase family.It plays a central role in centriole duplication and a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation.It has 3 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for PLK4 antibody 12952-1-APDownload protocol
IHC protocol for PLK4 antibody 12952-1-APDownload protocol
IF protocol for PLK4 antibody 12952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancers (Basel)

POLQ Overexpression Is Associated with an Increased Somatic Mutation Load and PLK4 Overexpression in Lung Adenocarcinoma.

Authors - Kazuya Shinmura

Eur Rev Med Pharmacol Sci

LncRNA SNHG1 contributes to tumorigenesis and mechanism by targeting miR-338-3p to regulate PLK4 in human neuroblastoma.

Authors - N Zhang

Int J Mol Med

MicroRNA‑126 suppresses the proliferation and migration of endothelial cells in experimental diabetic retinopathy by targeting polo‑like kinase 4.

Authors - Yarong Zheng

Int J Oncol

High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.

Authors - Zhibin Liao

Front Mol Biosci

Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.

Authors - Yongqu Lu

Mol Cell Biol

S6 Kinase- and β-TrCP2-Dependent Degradation of p19Arf Is Required for Cell Proliferation.

Authors - Tadashi Nakagawa