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PLK4 Polyclonal antibody
PLK4 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IHC, IF, ELISA
Cat no : 12952-1-AP
Validation Data Gallery
|Positive WB detected in||mouse lung tissue, MCF-7 cells, A549 cells|
|Positive IHC detected in||mouse testis tissue, human lung cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:25-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 3 publications below|
|WB||See 14 publications below|
|IHC||See 10 publications below|
|IF||See 5 publications below|
12952-1-AP targets PLK4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PLK4 fusion protein Ag3605|
|Full Name||polo-like kinase 4 (Drosophila)|
|Calculated molecular weight||970 aa, 109 kDa|
|Observed molecular weight||105-112 kDa|
|GenBank accession number||BC036023|
|Gene ID (NCBI)||10733|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PLK4(Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for PLK4 antibody 12952-1-AP||Download protocol|
|IHC protocol for PLK4 antibody 12952-1-AP||Download protocol|
|IF protocol for PLK4 antibody 12952-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Loss of KLF14 triggers centrosome amplification and tumorigenesis.
Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.
ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.
Cell Death Dis
Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.
Cell Death Dis
NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.
Mol Cancer Ther
Targeting Polo-like Kinase 1 by a Novel Pyrrole-imidazole Polyamide-Hoechst Conjugate Suppresses Tumor Growth in vivo.