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  • KD/KO Validated

PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


Human, Mouse and More (1)





Cat no : 28750-1-AP


PLK 4, PLK4, Polo like kinase 4, SAK, STK18

Tested Applications

Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28750-1-AP targets PLK4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLK4 fusion protein Ag30482
Full Name polo-like kinase 4 (Drosophila)
Calculated Molecular Weight 970 aa, 109 kDa
GenBank Accession NumberBC036023
Gene Symbol PLK4
Gene ID (NCBI) 10733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.


Product Specific Protocols
IHC protocol for PLK4 antibody 28750-1-APDownload protocol
IF protocol for PLK4 antibody 28750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biochem Biophys Res Commun

Advanced glycation end products initiate the mutual promoting cycle between centrosome amplification and the release of inflammatory cytokines in human vascular endothelial cells

Authors - Jun Zhang
  • KD Validated


Time-series reconstruction of the molecular architecture of human centriole assembly

Authors - Marine H Laporte


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Franziska (Verified Customer) (02-15-2023)

RPE1 WT cells were starved for 2 days. Fixed with -20°C Ethanol for 10 min at room temperature (RT), permeablized with 0.3% PBST(triton), blocked with 1%BSA in PBS for 1h at RT, PLK4 (rb, 1:50) and ARL13B (ms, 1:200) in 1% BSA in PBS diluted, prim AK Incubation for 1h at RT. Sek Ak goat anti rb (green) and goat anti mouse (pink). PLK4 can be found at the Basal Body of cilia and also stains cytoskeleton. PLK4 antibody could be tried with 1:200 dilution, to reduce background.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50, maybe 1:200 better
  • Cell Tissue Type: hTERT-RPE1
PLK4 Antibody Immunofluorescence validation (1:50, maybe 1:200 better dilution) in hTERT-RPE1 (Cat no:28750-1-AP)