PLK4 Polyclonal antibody
PLK4 Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
IHC, IF, ELISA
Cat no : 28750-1-AP
|Positive IHC detected in||human colon cancer tissue, mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
28750-1-AP targets PLK4 in IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
|Tested Reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PLK4 fusion protein Ag30482|
|Full Name||polo-like kinase 4 (Drosophila)|
|Calculated molecular weight||970 aa, 109 kDa|
|GenBank accession number||BC036023|
|Gene ID (NCBI)||10733|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Franziska (Verified Customer) (02-15-2023)
RPE1 WT cells were starved for 2 days. Fixed with -20°C Ethanol for 10 min at room temperature (RT), permeablized with 0.3% PBST(triton), blocked with 1%BSA in PBS for 1h at RT, PLK4 (rb, 1:50) and ARL13B (ms, 1:200) in 1% BSA in PBS diluted, prim AK Incubation for 1h at RT. Sek Ak goat anti rb (green) and goat anti mouse (pink). PLK4 can be found at the Basal Body of cilia and also stains cytoskeleton. PLK4 antibody could be tried with 1:200 dilution, to reduce background.