PMFBP1 Polyclonal antibody

PMFBP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17061-1-AP

Synonyms

PMF 1 binding protein, PMFBP1

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Validation Data Gallery

• 

  WB analysis of mouse testis using 17061-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17061-1-AP (PMFBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17061-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17061-1-AP (PMFBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17061-1-AP (PMFBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 2 publications below

Product Information

17061-1-AP targets PMFBP1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMFBP1 fusion protein Ag10789
• Full Name: polyamine modulated factor 1 binding protein 1
• Calculated Molecular Weight: 1022 aa, 119 kDa
• Observed Molecular Weight: 119 kDa
• GenBank Accession Number: BC028423
• Gene Symbol: PMFBP1
• Gene ID (NCBI): 83449
• RRID: AB_2299881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Nat Commun; The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus.; Authors - Ying Zhang
human	WB	Mol Genet Genomic Med; Novel mutations of PMFBP1 in a man with acephalic spermatozoa defects.; Authors - Hua Nie
mouse	WB	Alzheimers Res Ther; Spermidine/spermine-N1-acetyltransferase ablation impacts tauopathy-induced polyamine stress response.; Authors - Leslie A Sandusky-Beltran
human,mouse	IF	Clin Genet; Biallelic mutations in PMFBP1 cause acephalic spermatozoa.; Authors - Yan-Wei Sha