POC5 Polyclonal antibody

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  WB analysis using 22054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22054-1-AP (POC5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

POC5 Polyclonal Antibody for WB, ELISA

Cat No. 22054-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C5orf37, Centrosomal protein POC5

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22054-1-AP targets POC5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17060

  Product name: Recombinant human C5orf37 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 202-550 aa of BC101325

  Sequence: RKDEVISSLSHAIGKQKEKIELMRTFFHWRIGHVRARQDVYEGKLADQYYQRTLLKKVWKVWRSVVQKQWKDVVERACQARAEEVCIQISNDYEAKVAMLSGALENAKAEIQRMQHEKEHFEDSMKKAFMRGVCALNLEAMTIFQNRNDAGIDSTNNKKEEYGPGVQGKEHSAHLDPSAPPMPLPVTSPLLPSPPAAVGGASATAVPSAASMTSTRAASASSVHVPVSALGAGSAATAASEEMYVPRVVTSAQQKAGRTITARITGRCDFASKNRISSSLAIMGVSPPMSSVVVEKHHPVTVQTIPQATAAKYPRTIHPESSTSASRSLGTRSAHTQSLTSVHSIKVVD

• Full Name: chromosome 5 open reading frame 37
• Calculated Molecular Weight: 575 aa, 63 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC101325
• Gene Symbol: C5orf37
• Gene ID (NCBI): 134359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8NA72
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POC5 (POC5 Centriolar Protein) is a conserved protein essential for the assembly and structural integrity of the centriole, a core component of the centrosome and cilia. Localized to the distal portion of the centriole, it plays a critical role in centriole elongation, stabilization, and the maintenance of its characteristic nine-fold symmetric microtubule arrangement. By ensuring accurate centriole duplication, POC5 contributes to proper mitotic spindle formation and faithful chromosome segregation during cell division.