POLR3A Polyclonal antibody, PBS Only

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  WB analysis using 28842-1-AP (same clone as 28842-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 28842-1-AP (same clone as 28842-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

POLR3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 28842-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

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Product Information

28842-1-PBS targets POLR3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR3A fusion protein Ag29036
• Full Name: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• Calculated Molecular Weight: 1390 aa, 156 kDa
• Observed Molecular Weight: 155 kDa
• GenBank Accession Number: BC041089
• Gene Symbol: POLR3A
• Gene ID (NCBI): 11128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14802
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).