POU4F3 Polyclonal antibody

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  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21509-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

POU4F3 Polyclonal Antibody for WB, ELISA

Cat No. 21509-1-AP

Publications(9)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

Brain 3C, Brn 3C, BRN3C, DFNA15, POU class 4 homeobox 3, POU4F3

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Tested Applications

• Positive WB detected in: Neuro-2a cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

21509-1-AP targets POU4F3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16004

  Product name: Recombinant human POU4F3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 99-184 aa of BC104923

  Sequence: HPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDP

• Full Name: POU class 4 homeobox 3
• Calculated Molecular Weight: 338 aa, 37 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC104923
• Gene Symbol: POU4F3
• Gene ID (NCBI): 5459
• RRID: AB_2878872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
pig	IF	Adv Sci (Weinh); Injectable, Antioxidative, and Tissue-Adhesive Nanocomposite Hydrogel as a Potential Treatment for Inner Retina Injuries; Authors - Yi-Chen Liu
human	IF	Stem Cell Res Ther; Genetic correction of induced pluripotent stem cells from a DFNA36 patient results in morphologic and functional recovery of derived hair cell-like cells; Authors - Yi Luo
mouse	IHC	Front Cell Dev Biol; In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas.; Authors - Dongchang Xiao
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

AMRITA (Verified Customer) (02-15-2021)

The antibody requires a prior antigen retrieval step for 15min in 80 deg centigrade

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Inner ear - organ of Corti hair cells stained

POU4F3 Antibody Immunohistochemistry validation (1:200 dilution) in Inner ear - organ of Corti hair cells stained (Cat no:21509-1-AP)