PPARGC1B Polyclonal antibody

• Featured Product
• KD/KO Validated

PPARGC1B Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 22378-1-AP

Synonyms

ERRL1, PERC, PGC 1 beta, PGC 1(beta), PGC1, PGC1B, PPAR gamma coactivator 1 beta, PPARGC 1 beta, PPARGC1, PPARGC1B

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 22378-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22378-1-AP (PPARGC1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 22378-1-AP

  IP Result of anti-PPARGC1B (IP:22378-1-AP, 4ug; Detection:22378-1-AP 1:300) with mouse liver tissue lysate 4000ug.

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 22378-1-AP is PPARGC1B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: fish, Goat, human, Megalobrama amblycephala, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPARGC1B fusion protein Ag17566
• Full Name: peroxisome proliferator-activated receptor gamma, coactivator 1 beta
• Calculated molecular weight: 1023 aa, 113 kDa
• Observed molecular weight: 100-110 kDa
• GenBank accession number: BC132971
• Gene symbol: PPARGC1B
• Gene ID (NCBI): 133522
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	FEBS J; Mitochondrial metabolism is inhibited by the HIF1α-MYC-PGC-1β axis in BRAF V600E thyroid cancer.; Authors - Yanyan Gao
Goat	WB	J Anim Sci Biotechnol; miR-25 modulates triacylglycerol and lipid accumulation in goat mammary epithelial cells by repressing PGC-1beta.; Authors - Liuan Ma
Megalobrama amblycephala	WB	Comp Biochem Physiol C Toxicol Pharmacol; Carbonyl cyanide 3-chlorophenylhydrazone induced the imbalance of mitochondrial homeostasis in the liver of Megalobrama amblycephala: A dynamic study.; Authors - Ling Zhang
fish	WB	Br J Nutr; Interactions between dietary carbohydrate and thiamine: implications on the growth performance and intestinal mitochondrial biogenesis and function of Megalobrama amblycephala.; Authors - Chao Xu
pig	WB	Br J Nutr; Dietary leucine supplementation alters energy metabolism and induces slow-to-fast transitions in longissimus dorsi muscle of weanling piglets.; Authors - Qiwen Fan
Megalobrama amblycephala	WB	Front Physiol; Benfotiamine, a Lipid-Soluble Analog of Vitamin B1, Improves the Mitochondrial Biogenesis and Function in Blunt Snout Bream (Megalobrama amblycephala) Fed High-Carbohydrate Diets by Promoting the AMPK/PGC-1β/NRF-1 Axis.; Authors - Chao Xu