PPEF1 Polyclonal antibody, PBS Only

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  WB analysis using 12875-1-AP (same clone as 12875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12875-1-AP (PPEF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12875-1-AP (same clone as 12875-1-PBS)

  WB result of PPEF1 antibody (12875-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPEF1 transfected HeLa cells. This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 12875-1-AP (same clone as 12875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 12875-1-AP (same clone as 12875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12875-1-AP (PPEF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12875-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PPEF1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12875-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12875-1-PBS targets PPEF1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3553

  Product name: Recombinant human PPEF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC036026

  Sequence: MGCSSSSTKTRRSDTSLRAALIIQNWYRGYKARLKARQHYALTIFQSIEYADEQGQMQLSTFFSFMLENYTHIHKEELELRNQSLESEQDMRDRWDYVDSIDVPDSYNGPRLQFPLTCTDIDLLLEAFKEQQILHAHYVLEVLFETKKVLKQMPNFTHIQTSPSKEVTICGDLHGKLDDLFLIFYKNGLPSERNPYVFNGDFVDRGKNSIEILMILCVSFLVYPNDLHLNRGNHEDFMMNLRYGFTKEILHKYKLHGKRILQILEEFYAWLPIGTIVDNEILVIHGGISETTDLNLLHRVERNKMKSVLIPPTETNRDHDTDSKHNKVGVTFNAHGRIKTNGSPTEHLTE

• Full Name: protein phosphatase, EF-hand calcium binding domain 1
• Calculated Molecular Weight: 653 aa, 76 kDa
• Observed Molecular Weight: 69-76 kDa
• GenBank Accession Number: BC036026
• Gene Symbol: PPEF1
• Gene ID (NCBI): 5475
• RRID: AB_2166375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14829
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.