PPID Polyclonal antibody

PPID Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12716-1-AP

Synonyms

Cyclophilin 40, Cyclophilin D, Cyclophilin related protein, CYP 40, CYP40, CYPD, peptidylprolyl isomerase D, PPIase D, PPID, Rotamase D

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Validation Data Gallery

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  WB analysis of mouse brain using 12716-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12716-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12716-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12716-1-AP

  IP result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 12716-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PPID antibody (12716-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, human brain tissue, Jurkat cells, MCF-7 cells, NIH/3T3 cells, Raji cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

12716-1-AP targets PPID in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPID fusion protein Ag3409
• Full Name: peptidylprolyl isomerase D
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 38-41 kDa
• GenBank Accession Number: BC030707
• Gene Symbol: PPID
• Gene ID (NCBI): 5481
• RRID: AB_2237551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Publications

Species	Application	Title
rat	WB,CoIP	Cardiovasc Toxicol; PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice.; Authors - Tingting Wu
human	WB	Mol Med Rep; Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.; Authors - Da-Wei Liu
human	WB	Eur J Pharmacol; A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.; Authors - Yong-Cheng Ma
human	WB	Dev Cell; Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome; Authors - Ji Geng
rat	WB,IF	Bone Res; Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40); Authors - Junqin Li
mouse,rat	WB,CoIP	J Hazard Mater; T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway; Authors - Fang-Fang Yu