PPID Polyclonal antibody

• Home
• Products
• Primary Antibodies
• PPID Polyclonal antibody (12716-1-AP)

• KD/KO Validated

PPID Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12716-1-AP Copied!

Publications (18)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin 40, Cyclophilin-40, Cyclophilin-related protein, CYP40

• 

  WB analysis of MCF-7 using 12716-1-AP

  WB result of PPID antibody (12716-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPID transfected MCF-7 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 12716-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of NIH/3T3 using 12716-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat brain using 12716-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12716-1-AP

  IP result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of K-562 using 12716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PPID antibody (12716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for PPID in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, Jurkat cells, A549 cells, Raji cells, MCF-7 cells, NIH/3T3 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12716-1-AP targets PPID in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3409

  Product name: Recombinant human PPID protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 57-370 aa of BC030707

  Sequence: GIGHTTGKPLHFKGCPFHRIIKKFMIQGGDFSNQNGTGGESIYGEKFEDENFHYKHDREGLLSMANAGRNTNGSQFFITTVPTPHLDGKHVVFGQVIKGIGVARILENVEVKGEKPAKLCVIAECGELKEGDDGGIFPKDGSGDSHPDFPEDADIDLKDVDKILLITEDLKNIGNTFFKSQNWEMAIKKYAEVLRYVDSSKAVIETADRAKLQPIALSCVLNIGACKLKMSNWQGAIDSCLEALELDPSNTKALYRRAQGWQGLKEYDQALADLKKAQGIAPEDKAIQAELLKVKQKIKAQKDKEKAVYAKMFA

• Full Name: peptidylprolyl isomerase D
• Calculated Molecular Weight: 370 aa, 41 kDa
• Observed Molecular Weight: 38-41 kDa
• GenBank Accession Number: BC030707
• Gene Symbol: PPID
• Gene ID (NCBI): 5481
• RRID: AB_2237551
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q08752
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228). This protein should not be confused with mitochondrial peptidyl-prolyl cis-trans isomerase F (PPIF) which is often referred to as cyclophilin D or CypD (PMID: 23538482).

Publications

What published studies show

Anti-Cyclophilin D (PPIF) antibody, catalog number 12716-1-AP, has 18 citations published in journals including Nature Cell Biology, Developmental Cell, Bone Research, Experimental Molecular Medicine, Cell Communication and Signaling, Journal of Hazardous Materials, Biomaterials Research, Molecular Medicine, Antioxidants, Biomedical Pharmacotherapy, European Journal of Pharmacology, International Journal of Molecular Sciences, Journal of Periodontal Research, Molecules, Molecular Medicine Reports, Frontiers in Molecular Neuroscience, Cardiovascular Toxicology, and Brain Research. Research fields span mitochondrial biology, cancer, neurodegeneration, cardiovascular disease, inflammation, and metabolic disorders.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Cell Biol; Chaperone-mediated insertion of mitochondrial import receptor TOM70 protects against diet-induced obesity; Authors - Pedro Latorre-Muro
rat	WB,IF	Bone Res; Sensory nerves directly promote osteoclastogenesis by secreting peptidyl-prolyl cis-trans isomerase D (Cyp40); Authors - Junqin Li
human,mouse	WB,IF,CoIP	Exp Mol Med; Extracellular vesicle-mediated delivery of circp53 suppresses the progression of multiple cancers by activating the CypD/TRAP/HSP90 pathway.; Authors - Xichao Yu
human	CoIP	Cell Commun Signal; Mitochondrial DNA release via mPTP and BAX/BAK drives inflammatory injury in intestinal ischemia reperfusion.; Authors - Yixin Jing
rat,mouse	WB,CoIP	J Hazard Mater; T-2 toxin induces mitochondrial dysfunction in chondrocytes via the p53-cyclophilin D pathway; Authors - Fang-Fang Yu
human	WB,IHC	Biomater Res; Hyaluronic Acid-Functionalized Bismuth Vanadate/Molybdenum Disulfide Nanoheterojunctions Achieve Efficient Phototherapy of Hypoxic Tumor; Authors - Yunqing Pang